基于微型荧光显微镜技术对神经疾病中钙信号传导的见解

Calcium Signalling in Neurological Disorders, with Insights from Miniature Fluorescence Microscopy.

作者信息

Sun Dechuan, Amiri Mona, Meng Qi, Unnithan Ranjith R, French Chris

机构信息

Neural Dynamics Laboratory, Department of Medicine, The University of Melbourne, Melbourne, VIC 3052, Australia.

Department of Electrical and Electronic Engineering, The University of Melbourne, Melbourne, VIC 3052, Australia.

出版信息

Cells. 2024 Dec 25;14(1):4. doi: 10.3390/cells14010004.

DOI:10.3390/cells14010004

PMID:39791705

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11719922/

Abstract

Neurological disorders (NDs), such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and schizophrenia, represent a complex and multifaceted health challenge that affects millions of people around the world. Growing evidence suggests that disrupted neuronal calcium signalling contributes to the pathophysiology of NDs. Additionally, calcium functions as a ubiquitous second messenger involved in diverse cellular processes, from synaptic activity to intercellular communication, making it a potential therapeutic target. Recently, the development of the miniature fluorescence microscope (miniscope) enabled simultaneous recording of the spatiotemporal calcium activity from large neuronal ensembles in unrestrained animals, providing a novel method for studying NDs. In this review, we discuss the abnormalities observed in calcium signalling and its potential as a therapeutic target for NDs. Additionally, we highlight recent studies that utilise miniscope technology to investigate the alterations in calcium dynamics associated with NDs.

摘要

神经疾病（NDs），如肌萎缩侧索硬化症（ALS）、阿尔茨海默病（AD）、帕金森病（PD）、亨廷顿病（HD）和精神分裂症，是一项复杂且多方面的健康挑战，影响着全球数百万人。越来越多的证据表明，神经元钙信号紊乱促成了神经疾病的病理生理学过程。此外，钙作为一种普遍存在的第二信使，参与从突触活动到细胞间通讯等多种细胞过程，使其成为一个潜在的治疗靶点。最近，微型荧光显微镜（微型显微镜）的发展使得能够在不受束缚的动物体内同时记录大型神经元群体的时空钙活性，为研究神经疾病提供了一种新方法。在这篇综述中，我们讨论了在钙信号中观察到的异常情况及其作为神经疾病治疗靶点的潜力。此外，我们重点介绍了最近利用微型显微镜技术研究与神经疾病相关的钙动力学变化的研究。