Delice Nurcan, Ertugrul Tugrul, Tutuncu Serife, Ugurlu Mustafa, Uysal Akif
Faculty of Veterinary Medicine, Department of Histology and Embryology, Ondokuz Mayis University, Samsun, Turkey.
Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandry, Ondokuz Mayıs University, Samsun, Turkey.
Vet Med Sci. 2025 Jan;11(1):e70145. doi: 10.1002/vms3.70145.
Ration composition may significantly impact the nutrient absorption, duodenal parameters, intestinal health and feed efficiency of animals.
The objective of this study was to analyse the impact of concentrate- and forage-based diets on essential morphological parameters of the duodenum, including villus height, villus width, crypt depth and goblet cell density, in three different lamb breeds.
Forty-five lambs, aged between 2.5 and 3 months and with an average weight of around 20 kg, were selected. The lambs were fattened on two different diets consisting of pelleted concentrate and alfalfa hay in predetermined ratios: 70% concentrate + 30% forage and 70% forage + 30% concentrate. When the lambs reached 40 kg, they were slaughtered, and duodenum tissue samples were collected for analysis.
Histological characteristics were assessed by evaluating duodenal tissues from lambs of three breeds-Akkaraman, Karayaka and Herik-across the two dietary groups within each breed, and the essential parameters of the villus were measured. Akkaraman lambs exhibited lower goblet cell count and villus height (VH) as compared to Karayaka and Herik lambs. Goblet cell count and VH were higher in concentrate-fed lambs, except for Karayaka lambs. However, goblet cell density was similar across Akkaraman, Karayaka and Herik lambs. Herik lambs had higher VH, villus width (VW) and villus surface area as compared to Akkaraman and Karayaka. Villus parameters were generally lower in concentrate-fed lambs, except for Herik lambs.
Overall, the study suggests that altering the roughage-concentrate ratio did not significantly affect duodenal mucosa parameters. However, differences were observed across genotypes, particularly in Herik lambs, indicating potential associations between duodenal parameters and genotype.
日粮组成可能会显著影响动物的营养吸收、十二指肠参数、肠道健康和饲料效率。
本研究的目的是分析以精料和粗饲料为主的日粮对三种不同品种羔羊十二指肠的重要形态学参数的影响，这些参数包括绒毛高度、绒毛宽度、隐窝深度和杯状细胞密度。
选取45只年龄在2.5至3个月之间、平均体重约20千克的羔羊。这些羔羊以两种不同的日粮育肥，日粮由颗粒精料和苜蓿干草按预定比例组成：70%精料 + 30%粗饲料和70%粗饲料 + 30%精料。当羔羊体重达到40千克时，将它们屠宰，并采集十二指肠组织样本进行分析。
通过评估三个品种（阿卡拉曼、卡拉亚卡和赫里克）羔羊在两个日粮组中的十二指肠组织来评估组织学特征，并测量绒毛的重要参数。与卡拉亚卡和赫里克羔羊相比，阿卡拉曼羔羊的杯状细胞计数和绒毛高度（VH）较低。除卡拉亚卡羔羊外，精料喂养的羔羊杯状细胞计数和VH较高。然而，阿卡拉曼、卡拉亚卡和赫里克羔羊的杯状细胞密度相似。与阿卡拉曼和卡拉亚卡羔羊相比，赫里克羔羊的VH、绒毛宽度（VW）和绒毛表面积更高。除赫里克羔羊外, 精料喂养的羔羊绒毛参数通常较低。
总体而言，该研究表明改变粗饲料与精料的比例不会显著影响十二指肠黏膜参数。然而，在不同基因型之间观察到了差异，特别是在赫里克羔羊中，这表明十二指肠参数与基因型之间可能存在关联。
