Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
J Trace Elem Med Biol. 2022 Jan;69:126897. doi: 10.1016/j.jtemb.2021.126897. Epub 2021 Nov 4.
Selenium (Se) is an essential micronutrient that is important to both animals and humans. Furthermore, scientists are increasingly interested in boosting the Se content of food products for human consumption, such as Se-enriched meat, because it has been shown to have a positive effect on human health. The purpose of this study was to compare the effects of green synthesized nano selenium (GNS) on broiler chicken growth performance, meat selenium content, intestinal microflora, intestinal morphology, and immune response to sodium selenite (SS) as inorganic Se.
A total of 360 one-day-old Ross 308 broiler chickens were randomly assigned to four experimental groups, each of six replicates of 15 birds. The experimental treatments were as follows: (1) based diet supplemented with 0.15 mg/kg of SS (control group), (2-4) basal diet supplemented with 0.075, 0.15 and 0.3 mg/kg of GNS respectively.
The results showed that different experimental diets had no significant effect on growth performance. Meat Se content increased with dietary GNS supplementation (P < 0.05). The birds fed dietary supplements GNS compared to SS had higher lactic acid bacteria counts and lactic acid bacteria/coliform ratios in ileum on day 42 (P < 0.05). Furthermore, as compared to SS, dietary supplementation of 0.3 mg GNS/kg significantly reduced coliform numbers in the cecum on day 42 (P < 0.05). Supplementation of 0.3 mg GNS/kg in the diet vs. SS at 21 days, there was a significant increase in VH/CD in the ileum and jejunum, as well as villus height and villus surface area in the ileum, and a decrease in crypt depth and epithelial cell layer thickness in the jejunum. Furthermore, at 42 days, birds fed 0.3 mg GNS/kg had higher villus height, villus surface area, and goblet cell density in the ileum and jejunum than birds fed SS (P < 0.05). Compared to SS, broilers fed 0.3 mg GNS/kg dietary supplementation had higher IgG at 42 days (P < 0.05).
In comparison to the use of SS, it could be concluded that the inclusion of GNS as a novel Se source can improve meat Se content, intestinal microflora, intestinal morphology, and immune response.
硒（Se）是一种对动物和人类都很重要的必需微量元素。此外，科学家们越来越有兴趣提高人类食用的食品产品中的硒含量，例如富硒肉，因为它已被证明对人类健康有积极影响。本研究的目的是比较绿色合成纳米硒（GNS）对肉鸡生长性能、肉硒含量、肠道微生物群、肠道形态和对亚硒酸钠（SS）作为无机硒的免疫反应的影响。
将 360 只 1 日龄罗斯 308 肉鸡随机分为四组，每组 6 个重复，每个重复 15 只鸡。实验处理如下：（1）基础日粮添加 0.15mg/kg SS（对照组），（2-4）基础日粮分别添加 0.075、0.15 和 0.3mg/kg GNS。
结果表明，不同的实验日粮对生长性能没有显著影响。随着日粮 GNS 补充，肉硒含量增加（P<0.05）。与 SS 相比，日粮添加 GNS 的鸡在第 42 天回肠中乳酸菌数量和乳酸菌/大肠杆菌比例更高（P<0.05）。此外，与 SS 相比，日粮中添加 0.3mg GNS/kg 可显著降低第 42 天盲肠中的大肠杆菌数量（P<0.05）。与 21 天相比，日粮中添加 0.3mg GNS/kg 可显著增加回肠和空肠的 VH/CD、回肠的绒毛高度和绒毛表面积，并降低空肠的隐窝深度和上皮细胞层厚度。此外，42 天时，与 SS 相比，日粮中添加 0.3mg GNS/kg 的鸡回肠和空肠的绒毛高度、绒毛表面积和杯状细胞密度更高（P<0.05）。与 SS 相比，日粮中添加 0.3mg GNS/kg 的鸡在第 42 天的 IgG 更高（P<0.05）。
与使用 SS 相比，添加 GNS 作为新型硒源可以提高肉硒含量、肠道微生物群、肠道形态和免疫反应。
