Kozinets G I, Ivanova L E, Kuznetsova T V, Khanykova O K
Eksp Onkol. 1985;7(1):48-52.
A comparative quantitative investigation of colony-forming ability of mouse bone marrow hemopoietic stem cells in the case of ordinary leukemia variant (P-388) and carminomycin-resistant variant (P-388/k) was carried out. Leukemia bearing mice received single injections of increasing doses of carminomycin on the 5th day after transplantation and 24 hours later the bone marrow was investigated for quantity of colony-forming units in the spleen (CFUs). Dynamics investigation of bone marrow cells in the case of P-388 and P-388/k (without treatment) reveals the common tendency to a decrease of these cells in the period before animal death. Sensitivity of colony forming cells (CFUs) to different doses of the antibiotic of mouse bone marrow in both cases (P-388 and P-388/k) was practically the same. Dynamics of CFUs in two populations of cells after a single injection of carminomycin in doses causing the killing of 50% of CFUs showed that the antibiotic inhibited CFUs equally (50%). However this inhibition in case of CFUs with P-388/k leukemia was almost five times longer than in the case of ordinary P-388 one. Based on the investigations conducted the second injection of the antibiotic to mice with P-388/k leukemia on the fifteenth day after the first one may be recommended.
对普通白血病变种（P - 388）和耐卡米诺霉素变种（P - 388/k）小鼠骨髓造血干细胞的集落形成能力进行了比较定量研究。荷瘤小鼠在移植后第5天接受单次递增剂量的卡米诺霉素注射，24小时后检测骨髓中脾集落形成单位（CFUs）的数量。对P - 388和P - 388/k（未治疗）小鼠骨髓细胞的动态研究表明，在动物死亡前这段时间内，这些细胞有共同的减少趋势。在两种情况下（P - 388和P - 388/k），集落形成细胞（CFUs）对不同剂量小鼠骨髓抗生素的敏感性基本相同。单次注射导致50%的CFUs死亡剂量的卡米诺霉素后，两个细胞群体中CFUs的动态变化表明，该抗生素对CFUs的抑制作用相同（50%）。然而，对于患有P - 388/k白血病的CFUs，这种抑制作用的持续时间几乎是普通P - 388的五倍。基于所进行的研究，建议在首次注射后第15天对患有P - 388/k白血病的小鼠进行第二次抗生素注射。
