Miyanomae T, Okabe T, Mori K J, Seto A
Biomed Pharmacother. 1982;36(8-9):348-53.
Effects of induced salmonella infection on hemopoietic stem cells were studied by comparing hemopoietic response of mice to killed and viable bacteria of an attenuated strain of Salmonella enteritidis. Results are as follows: i) A single injection with viable bacteria induced an increase in pluripotent stem cells (CFUc) and granulocyte-macrophage progenitor cells (CFUs) in the spleen and a decrease in these cells in the bone marrow, ii) A single injection or repeated injections of killed bacteria resulted in an increase of CFUs and CFUs in the bone marrow as well as in the spleen, iii) Histological examination revealed no inflammatory change that might account for the different hemopoietic responses seen with the viable and killed bacteria. Our results suggest that the hemopoietic responses in salmonella-infected mice reflect not only the influence of LPS but also effects of viable intracellular bacteria on the reticuloendothelial system constituting the hemopoietic microenvironment.
通过比较小鼠对肠炎沙门氏菌减毒株的死菌和活菌的造血反应，研究了诱导沙门氏菌感染对造血干细胞的影响。结果如下：i）单次注射活菌可导致脾脏中多能干细胞（CFUc）和粒细胞-巨噬细胞祖细胞（CFUs）增加，而骨髓中这些细胞减少；ii）单次注射或重复注射死菌可导致骨髓和脾脏中CFUs和CFUs增加；iii）组织学检查未发现可能解释活菌和死菌所见不同造血反应的炎症变化。我们的结果表明，沙门氏菌感染小鼠的造血反应不仅反映了LPS的影响，还反映了活的细胞内细菌对构成造血微环境的网状内皮系统的影响。
