Ge Linyan, Liu Xiu, Zhang Lingyan, Zhang Jiaren, Song Guanghui
Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, NO. 3 Qingchun East Road, Hangzhou, 310016, China.
Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, School of Medicine, Zhejiang University, Hangzhou, China.
Eur J Med Res. 2025 Jan 10;30(1):21. doi: 10.1186/s40001-024-02232-5.
Ovarian cancer (OC) is a prevalent gynecological malignancy with a relatively dismal prognosis. The SGT1 homolog (SUGT1) protein, which interacts with heat shock protein 90 and is essential for the G1/S and G2/M transitions, was formerly thought to be a cancer promoter, but its precise role in OC remains unknown.
We conducted a comprehensive bioinformatics analysis of SUGT1 expression in patients with OC compared with their normal controls, including the data from the cancer genome atlas (TCGA), genotype-tissue expression (GTEx) databases, gene ontology (GO) analysis, Kyoto Encylopedia of Genes and Genomes (KEGG) analysis, gene set enrichment analysis (GSEA), single sample gene set enrichment analysis (ssGSEA). In addition, Kaplan-Meier (KM) analysis, univariate and multivariate Cox analyses were applied to investigate the prognostic role of SUGT1 in ovarian cancer. Furthermore, we validated the expression of SUGT1 in OC and normal tissues through immunohistochemistry.
SUGT1 was significantly overexpressed in OC than in normal tissues. In addition, the GO, KEGG and GSEA analysis revealed that SUGT1 was associated with the functions related to immunoglobulin complex, antigen binding, immunoglobulin receptor binding, among others. Besides, ssGSEA demonstrated a positive correlation between SUGT1 expression and the abundance of T central memory cells, natural killer cells, and T gamma delta cells, although it showed a negative association with activated dendritic cells, cytotoxic cells, T cells, and T helper 1 cells. Subsequently, KM survival analysis revealed that high SUGT1 expression indicated a shorter overall survival, disease specific survival and progression free interval in OC patients. Univariate and multivariate Cox regression revealed that SUGT1 could serve as an independent risk factor for prognosis of patients with OC.
All these results of this study show that SUGT1 is an important molecular component in immune infiltration in OC and may have a new significant prognostic role in OC.
卵巢癌（OC）是一种常见的妇科恶性肿瘤，预后相对较差。SGT1同源蛋白（SUGT1）与热休克蛋白90相互作用，对G1/S期和G2/M期转换至关重要，以前被认为是癌症促进因子，但其在OC中的具体作用仍不清楚。
我们对OC患者与正常对照者的SUGT1表达进行了全面的生物信息学分析，包括来自癌症基因组图谱（TCGA）、基因型-组织表达（GTEx）数据库的数据，基因本体（GO）分析、京都基因与基因组百科全书（KEGG）分析、基因集富集分析（GSEA）、单样本基因集富集分析（ssGSEA）。此外，应用Kaplan-Meier（KM）分析、单因素和多因素Cox分析来研究SUGT1在卵巢癌中的预后作用。此外，我们通过免疫组织化学验证了SUGT1在OC和正常组织中的表达。
SUGT1在OC中显著高于正常组织表达。此外，GO、KEGG和GSEA分析显示，SUGT1与免疫球蛋白复合物、抗原结合、免疫球蛋白受体结合等相关功能有关。此外，ssGSEA显示SUGT1表达与T中央记忆细胞、自然杀伤细胞和Tγδ细胞的丰度呈正相关，尽管它与活化树突状细胞、细胞毒性细胞、T细胞和T辅助1细胞呈负相关。随后，KM生存分析显示，高SUGT1表达表明OC患者的总生存期、疾病特异性生存期和无进展生存期较短。单因素和多因素Cox回归显示，SUGT1可作为OC患者预后的独立危险因素。
本研究的所有这些结果表明，SUGT1是OC免疫浸润中的重要分子成分，可能在OC中具有新的重要预后作用。
