Tan Shenluan, Zeng Fanwen, Zhong Wanhuan, Chen Tanzipeng, Chen Xuanjiao, Li Li, Wei Hengxi, Zhang Shouquan
State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510075, China.
Animals (Basel). 2024 Dec 24;15(1):7. doi: 10.3390/ani15010007.
Flamingos () are among the oldest birds worldwide and are loved by people for their bright red feathers. In addition, flamingos are sexually monomorphic birds, and distinguishing between males and females is difficult. The polymerase chain reaction (PCR) is widely used for sex identification. However, the PCR method requires a precise thermal cycler in the laboratory and is time-consuming. Therefore, developing a rapid, sensitive, and accurate method to identify the sex of flamingos is crucial. In this study, we established a sex identification system using a recombinase-aided amplification- Argonaute (RAA-Ago) technique for greater flamingo (). The greater flamingo-RAA-Ago system can identify unknown-sex greater flamingos in less than 1 h and can be visualized using a fluorescent detector or blue light. The results showed that optimal RAA-Ago conditions could detect 0.6 ng of genomic DNA and effectively differentiate between males and females. Random sample evaluations revealed that the system had a 100% coincidence rate compared with conventional PCR. In conclusion, this study provides a sensitive, specific, and accurate reference method for greater flamingo sexing.
火烈鸟是世界上最古老的鸟类之一，因其鲜艳的红色羽毛而深受人们喜爱。此外，火烈鸟是两性同形的鸟类，很难区分雌雄。聚合酶链反应（PCR）被广泛用于性别鉴定。然而，PCR方法在实验室中需要精确的热循环仪，且耗时较长。因此，开发一种快速、灵敏且准确的火烈鸟性别鉴定方法至关重要。在本研究中，我们建立了一种使用重组酶辅助扩增-Argonaute（RAA-Ago）技术的性别鉴定系统用于大火烈鸟。大火烈鸟-RAA-Ago系统可以在不到1小时的时间内鉴定出性别未知的大火烈鸟，并且可以使用荧光检测器或蓝光进行可视化。结果表明，最佳的RAA-Ago条件可以检测到0.6 ng的基因组DNA，并有效区分雌雄。随机样本评估显示，该系统与传统PCR相比符合率为100%。总之，本研究为大火烈鸟性别鉴定提供了一种灵敏、特异且准确的参考方法。
