Hubei Province Key Laboratory of Occupational Hazard identification and Control, School of Medicine, School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, PR China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, PR China.
ACS Sens. 2023 Oct 27;8(10):3623-3642. doi: 10.1021/acssensors.3c01232. Epub 2023 Oct 11.
Over the past few decades, pathogens have posed a threat to human security, and rapid identification of pathogens should be one of the ideal methods to prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive and specific approaches to identify and quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems and Argonaute (Ago) belong to the Microbial Defense Systems (MDS). The guided, programmable, and targeted activation of nucleases by both of them is leading the way to a new generation of pathogens detection. We compare these two nucleases in terms of similarities and differences. In addition, we discuss future challenges and prospects for the development of the CRISPR/Cas systems and Argonaute (Ago) biosensors, especially electrochemical biosensors. This review is expected to afford researchers entering this multidisciplinary field useful guidance and to provide inspiration for the development of more innovative electrochemical biosensors for pathogens detection.
在过去的几十年中，病原体对人类安全构成了威胁，快速鉴定病原体应该是预防重大公共卫生安全事件的理想方法之一。因此，迫切需要高度敏感和特异的方法来鉴定和定量病原体。成簇规律间隔短回文重复 CRISPR/Cas 系统和 Argonaute（Ago）属于微生物防御系统（MDS）。它们都可以引导、编程和靶向激活核酸酶，引领着新一代病原体检测的发展。我们比较了这两种核酸酶的异同。此外，我们还讨论了 CRISPR/Cas 系统和 Argonaute（Ago）生物传感器，特别是电化学生物传感器的未来挑战和发展前景。这篇综述有望为进入这个多学科领域的研究人员提供有用的指导，并为开发更多用于病原体检测的创新性电化学生物传感器提供灵感。
