Yu Jianing, Wu Biao, Dong Yinghui, Lin Zhihua, Yao Hanhan
College of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo 315101, China.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Animals (Basel). 2024 Dec 30;15(1):64. doi: 10.3390/ani15010064.
The razor clam , a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which belong to the NAD(P)-dependent superfamily, play a crucial role in stress resilience by participating in catabolic and anabolic pathways, such as carnitine synthesis, glycolysis, and amino acid metabolism. This study presents the first comprehensive analysis of the ALDH family in under acute high salt stress conditions and identifies 16 genes across 10 subfamilies. These genes are located on eight chromosomes, with tandem duplications observed on chromosome 10; they encode mostly acidic and hydrophilic proteins. Among them, contains a conserved P5CS domain that is implicated in proline synthesis and osmotic regulation. The expression of 14 members were significantly altered under acute salt stress conditions, with and showing increased expression levels, suggesting their involvement in osmotic pressure regulation. This research provides insights into the characteristics, evolution, and response to salinity stress of the gene family while shedding light on ALDH function in bivalves, as well as serving as a foundation for further studies on osmotic stress regulation.
缢蛏是一种重要的海洋双壳贝类，栖息于河口地区，会遭遇盐度胁迫。尽管其具有商业重要性，但人们对其适应高盐度的机制了解有限。醛脱氢酶（ALDHs）属于NAD(P)依赖超家族，通过参与分解代谢和合成代谢途径，如肉碱合成、糖酵解和氨基酸代谢，在应激恢复中发挥关键作用。本研究首次对缢蛏在急性高盐胁迫条件下的ALDH家族进行了全面分析，鉴定出了10个亚家族中的16个基因。这些基因位于8条染色体上，在10号染色体上观察到串联重复；它们大多编码酸性和亲水性蛋白质。其中，有一个保守的P5CS结构域，与脯氨酸合成和渗透调节有关。在急性盐胁迫条件下，14个基因成员的表达发生了显著变化，其中两个基因的表达水平升高，表明它们参与渗透压调节。本研究为缢蛏ALDH基因家族的特征、进化和对盐度胁迫的响应提供了见解，同时揭示了双壳贝类中ALDH的功能，并为进一步研究渗透胁迫调节奠定了基础。
