Bebianno M J, Sroda S, Gomes T, Chan P, Bonnafe E, Budzinski H, Geret F
CIMA, University of Algarve, Campus Gambelas, 8005-139, Faro, Portugal.
Laboratory of Alpine Ecology - LECA UMR-CNRS 5553, Equipe P3E 2233, rue de la piscine Campus Universitaire de Grenoble, 38400, Saint Martin d'Hères, France.
Environ Sci Pollut Res Int. 2016 Mar;23(6):5046-55. doi: 10.1007/s11356-015-5395-5. Epub 2015 Oct 1.
The increase use of pharmaceutical compounds in veterinary practice and human population results in the ubiquitous presence of these compounds in aquatic ecosystems. Because pharmaceuticals are highly bioactive, there is concern about their toxicological effects in aquatic organisms. Therefore, the aim of this study was to assess the effects of an effluent from a psychiatric hospital (containing a complex mixture of 25 pharmaceutical compounds from eleven therapeutic classes) on the freshwater clam Corbicula fluminea using a proteomic approach. The exposure of C. fluminea to this complex effluent containing anxiolytics, analgesics, lipid regulators, beta blockers, antidepressants, antiepileptics, antihistamines, antihypertensives, antiplatelets and antiarrhythmics induced protein changes after 1 day of exposure in clam gills and digestive gland more evident in the digestive gland. These changes included increase in the abundance of proteins associated with structural (actin and tubulin), cellular functions (calreticulin, proliferating cell nuclear antigen (PCNA), T complex protein 1 (TCP1)) and metabolism (aldehyde dehydrogenase (ALDH), alcohol dehydrogenase, 6 phosphogluconate dehydrogenase). Results from this study indicate that calreticulin, PCNA, ALDH and alcohol dehydrogenase in the digestive gland and T complex protein 1 (TCP1)) and 6 phosphogluconate dehydrogenase in the gills represent useful biomarkers for the ecotoxicological characterization of psychiatric hospital effluents in this species.
兽医学实践和人类群体中药物化合物使用的增加，导致这些化合物在水生生态系统中普遍存在。由于药物具有高度生物活性，人们担心它们对水生生物的毒理学影响。因此，本研究的目的是使用蛋白质组学方法评估一家精神病医院的废水（含有来自11个治疗类别的25种药物化合物的复杂混合物）对淡水蚬Corbicula fluminea的影响。将C. fluminea暴露于这种含有抗焦虑药、镇痛药、脂质调节剂、β受体阻滞剂、抗抑郁药、抗癫痫药、抗组胺药、抗高血压药、抗血小板药和抗心律失常药的复杂废水中，暴露1天后，在蚬的鳃和消化腺中诱导了蛋白质变化，在消化腺中更为明显。这些变化包括与结构（肌动蛋白和微管蛋白）、细胞功能（钙网蛋白、增殖细胞核抗原（PCNA）、T复合体蛋白1（TCP1））和代谢（醛脱氢酶（ALDH）、乙醇脱氢酶、6-磷酸葡萄糖酸脱氢酶）相关的蛋白质丰度增加。本研究结果表明，消化腺中的钙网蛋白、PCNA、ALDH和乙醇脱氢酶以及鳃中的T复合体蛋白1（TCP1）和6-磷酸葡萄糖酸脱氢酶是该物种精神病医院废水生态毒理学特征的有用生物标志物。
