Cañadas-Vidal Esmeralda, Muñoz-Prieto Alberto, Rešetar Maslov Dina, Rubić Ivana, González-Sánchez Juan C, Garcia-Martinez Juan D, Ceron José J, Mrljak Vladimir, Pardo-Marin Luis, Martinez-Subiela Silvia, Tvarijonaviciute Asta
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, Espinardo, 30100 Murcia, Spain.
Veterinary Clinical Hospital, University of Murcia, Calle Campus Universitario 16, Espinardo, 30100 Murcia, Spain.
Animals (Basel). 2025 Jan 3;15(1):91. doi: 10.3390/ani15010091.
Obesity is defined as the excessive accumulation of adipose tissue and is currently the most common disease in cats. Similarly to humans, obesity negatively impacts the health and welfare of cats, predisposing them to many other disorders. The objective of this study was to compare the serum proteomes of normal-weight and overweight/obese cats, aiming to gain insights into the physiopathology of feline obesity and potentially identify new biomarkers. For this, serum samples from a total of 20 adult neutered domestic shorthair client-owned cats, ten normal weight and ten overweight/obese, were submitted to tandem mass tags labelling and liquid chromatography-mass spectrometry (LC-MS/MS) analysis. A total of 288 proteins were detected in the serum samples. Out of these, 12 proteins showed statistically significant differences in abundance between control cats and cats with obesity, namely Ig-like domain-containing protein, Alpha-2-HS-glycoprotein, Complement C8 gamma chain, An-tithrombin-III, Serpin family A member 1, Complement factor H, C3-beta-c, Albumin, C4b-binding protein alpha chain, Alpha-1-B glycoprotein, Solute carrier family 12 member 4, and Fibronectin. Overall this report identifies new proteins involved and provides additional knowledge about the physiopathological changes related to feline obesity.
肥胖被定义为脂肪组织过度蓄积，目前是猫最常见的疾病。与人类相似，肥胖对猫的健康和福祉有负面影响，使它们易患许多其他疾病。本研究的目的是比较正常体重和超重/肥胖猫的血清蛋白质组，旨在深入了解猫肥胖的生理病理学，并有可能识别新的生物标志物。为此，从总共20只成年绝育家养短毛客户拥有的猫（10只正常体重和10只超重/肥胖）采集血清样本，进行串联质量标签标记和液相色谱-质谱联用（LC-MS/MS）分析。血清样本中共检测到288种蛋白质。其中，12种蛋白质在对照猫和肥胖猫之间的丰度存在统计学显著差异，即含免疫球蛋白样结构域蛋白、α-2-HS-糖蛋白、补体C8γ链、抗凝血酶III、丝氨酸蛋白酶抑制剂家族A成员1、补体因子H、C3-β-c、白蛋白、C4b结合蛋白α链、α-1-B糖蛋白、溶质载体家族12成员4和纤连蛋白。总体而言，本报告确定了涉及的新蛋白质，并提供了有关猫肥胖相关生理病理变化的更多知识。
