Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark.
Centre for Companion Animal Health and Immunology and Metabolic Medicine, University of Queensland, Brisbane, Queensland, Australia.
Domest Anim Endocrinol. 2014 Apr;47:55-64. doi: 10.1016/j.domaniend.2013.11.006. Epub 2013 Dec 5.
In this study, we estimated insulin sensitivity and determined plasma concentrations of total-, low-molecular-weight (LMW), and high-molecular-weight (HMW) adiponectin and leptin in 72 domestic shorthair, neutered, client-owned cats. Glucose tolerance was assessed with an intravenous glucose tolerance test and body fat percentage (BF%) was measured with dual-energy x-ray absorptiometry. Total adiponectin was measured with 2 different ELISAs. Low-molecular-weight and HMW adiponectin plasma concentrations were determined by Western blot analysis after sucrose-gradient velocity centrifugation, and the adiponectin multimer ratio [SA = HMW/(HMW + LMW)] was calculated. Differences in glucose tolerance, leptin, total adiponectin, and multimer ratio among lean (BF% <35; n = 26), overweight (35 <BF% <45; n = 28), and obese (BF% >45; n = 18) cats as well as between male (n = 34) and female (n = 38) neutered cats were evaluated by linear regression and 2-way ANOVA. Sex and age were included as covariates for analysis of BF%, whereas BF%, fat mass, and lean body mass were covariates for analysis of sex differences. Increased BF% was negatively correlated with multimer ratio (SA, r = -45; P < 0.002), whereas no differences were found in total adiponectin concentrations among BF% groups (P > 0.01). Male cats had indices of decreased insulin tolerance and significantly lower total adiponectin concentrations than did female cats (mean ± SEM, 3.7 ± 0.4 vs 5.4 ± 0.5 μg/mL; P < 0.02). Altered SAs could contribute to an obesity-associated decreasing glucose tolerance in cats, and low total adiponectin concentrations may relate to increased risk of diabetes mellitus in neutered male cats.
在这项研究中，我们评估了 72 只绝育的家养短毛猫的胰岛素敏感性，并测定了总、低分子量 (LMW) 和高分子量 (HMW) 脂联素和瘦素的血浆浓度。葡萄糖耐量通过静脉葡萄糖耐量试验进行评估，体脂肪百分比 (BF%) 通过双能 X 射线吸收法进行测量。总脂联素使用 2 种不同的 ELISA 进行测量。LMW 和 HMW 脂联素的血浆浓度通过蔗糖梯度速度离心后的 Western blot 分析确定，并计算了脂联素多聚体比 [SA = HMW/(HMW + LMW)]。通过线性回归和 2 因素方差分析评估了瘦体（BF% < 35；n = 26）、超重（35 < BF% < 45；n = 28）和肥胖（BF% > 45；n = 18）猫之间以及雄性（n = 34）和雌性（n = 38）绝育猫之间葡萄糖耐量、瘦素、总脂联素和多聚体比的差异。性别和年龄被纳入 BF%分析的协变量，而 BF%、脂肪量和瘦体量被纳入性别差异分析的协变量。增加的 BF%与多聚体比（SA）呈负相关（r = -45；P < 0.002），而 BF%组之间的总脂联素浓度没有差异（P > 0.01）。雄性猫的胰岛素耐受性指数降低，且总脂联素浓度明显低于雌性猫（平均值 ± SEM，3.7 ± 0.4 对 5.4 ± 0.5 μg/mL；P < 0.02）。改变的 SAs 可能导致猫肥胖相关的葡萄糖耐量降低，而总脂联素浓度低可能与绝育雄性猫患糖尿病的风险增加有关。
