Liu Shaoshuai, Yan Wenjuan, Ma Chang, Liu Yajing, Gong Limin, Levesque Crystal, Dong Bing
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Zhejiang BioAsia Biomedical Co., Ltd., Pinghu, Zhejiang, 314200, China.
Asian-Australas J Anim Sci. 2020 Apr;33(4):568-578. doi: 10.5713/ajas.19.0179. Epub 2019 Aug 3.
The objective of this study was to investigate effects of supplementation of culture media from solid-state fermented Isaria cicadae (I. cicadae) on performance, serum biochemical parameters, serum immune indexes, antioxidant capacity and meat quality of broiler chickens.
A total of 648 Arbor Acres male broiler chickens(1 d; average body weight, 42.93± 0.47 g) were randomly assigned to 6 treatments, each with six replicates and 18 broiler chickens per replicate. Broiler chickens were fed phase I (d 1 to 21) and phase II (d 22 to 42) diets. The phase I diets were corn and soybean-meal based diets supplemented with 0%, 2%, 4%, 6%, 8%, or 10% culture media from solid-state fermented I. cicadae respectively. The phase II diets were corn and soybean-meal based diets supplemented with 0%, 1.33%, 2.67%, 4.00%, 5.32%, or 6.67% culture media from solid-state fermented I. cicadae respectively.
In phase I, the broiler chickens with the supplementation of culture media had increased body weight gain and feed intake (linear and quadratic, p<0.05) with increasing inclusion of culture media. The levels of serum total antioxidant capacity (T-AOC), glutathione (GSH) and superoxide dismutase (SOD) increased linearly (p<0.05). In phase II, levels of serum T-AOC and interleukin-1β increased linearly (p<0.05), and GSH increased (p<0.05). In the kidney, GSH and glutathione peroxidase (GSH-Px) concentrations increased (linear and quadratic, p<0.05) and SOD concentration increased linearly (p<0.05). Compared to the control, shear force and drip loss of breast muscle decreased (linear and quadratic, p<0.05). Drip loss of leg muscle decreased linearly and quadratically (p<0.05).
Dietary supplementation of culture media from solid-state fermented I.cicadae which was enriched in both wheat and residual bioactive components of I. cicadae enhanced the growth performance of broiler chickens. It also improved body anti-oxidative status and contributed to improve broiler meat quality.
本研究旨在探讨添加固态发酵蝉花培养基对肉鸡生长性能、血清生化参数、血清免疫指标、抗氧化能力及肉质的影响。
选取648只1日龄爱拔益加雄性肉鸡（平均体重42.93±0.47 g），随机分为6组，每组6个重复，每个重复18只肉鸡。肉鸡分别饲喂121日龄和2242日龄的日粮。121日龄日粮是以玉米和豆粕为基础的日粮，分别添加0%、2%、4%、6%、8%或10%的固态发酵蝉花培养基。2242日龄日粮是以玉米和豆粕为基础的日粮，分别添加0%、1.33%、2.67%、4.00%、5.32%或6.67%的固态发酵蝉花培养基。
在121日龄阶段，随着培养基添加量的增加，添加培养基的肉鸡体重增加和采食量增加（线性和二次曲线，p<0.05）。血清总抗氧化能力（T-AOC）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）水平呈线性增加（p<0.05）。在2242日龄阶段，血清T-AOC和白细胞介素-1β水平呈线性增加（p<0.05），GSH增加（p<0.05）。在肾脏中，GSH和谷胱甘肽过氧化物酶（GSH-Px）浓度增加（线性和二次曲线，p<0.05），SOD浓度呈线性增加（p<0.05）。与对照组相比，胸肌的剪切力和滴水损失降低（线性和二次曲线，p<0.05）。腿肌的滴水损失呈线性和二次曲线降低（p<0.05）。
日粮中添加富含小麦和蝉花残留生物活性成分的固态发酵蝉花培养基可提高肉鸡的生长性能，改善机体抗氧化状态，并有助于提高鸡肉品质。
