Machida Koichi, Kotani Akira, Osaki Tomoya, Kobayashi Ayaka, Yamamoto Kazuhiro, Hakamata Hideki
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji 192-0392, Japan.
Molecules. 2024 Dec 26;30(1):48. doi: 10.3390/molecules30010048.
The appearances of Schisandrae Sphenantherae Fructus (SSF) and Schisandrae Chinensis Fructus (SCF) are very similar. Thus, being able to distinguish between SSF and SCF is useful for the quality control of these herbal medicines. In this study, two kinds of electrochemical fingerprint analyses using voltammetry or HPLC with electrochemical detection (HPLC-ECD) were developed in combination with multivariate analysis for discriminating between SSF and SCF. The data sets of the oxidation current values from voltammograms of SSF and SCF samples ranging from +0.5 to +1.0 V were applied to perform a partial least squares discriminant analysis (PLS-DA). Moreover, the data sets of the current heights of the characteristic target peaks on the chromatograms at an applied potential of +0.9 V were also applied to perform PLS-DA. In each PLS-DA obtained from the voltammograms and chromatograms, the scores for the SSF samples were plotted on a different region compared with the scores for the SCF samples. Considering the results of the cross-validation, the HPLC-ECD clearly discriminated between the SSF and SCF samples when compared with the voltammetry. In conclusion, our results show that the present electrochemical fingerprint analyses coupled with PLS-DA are useful as a means for discriminating between the SSF and SCF samples.
南五味子和五味子的外观非常相似。因此，能够区分南五味子和五味子对于这些草药的质量控制很有用。在本研究中，开发了两种使用伏安法或高效液相色谱-电化学检测（HPLC-ECD）的电化学指纹分析方法，并结合多变量分析来区分南五味子和五味子。将南五味子和五味子样品伏安图中从+0.5至+1.0 V的氧化电流值数据集用于进行偏最小二乘判别分析（PLS-DA）。此外，在+0.9 V施加电位下色谱图上特征目标峰的电流高度数据集也用于进行PLS-DA。在从伏安图和色谱图获得的每个PLS-DA中，与五味子样品的得分相比，南五味子样品的得分绘制在不同区域。考虑到交叉验证的结果，与伏安法相比，HPLC-ECD能明显区分南五味子和五味子样品。总之，我们的结果表明，目前的电化学指纹分析结合PLS-DA可作为区分南五味子和五味子样品的一种手段。
