Gutierrez-Montiel Daniela, Guerrero-Barrera Alma L, Ramírez-Castillo Flor Y, Galindo-Guerrero Fabiola, Ornelas-García Ingrid G, Chávez-Vela Norma A, de O Costa Matheus, Avelar-Gonzalez Francisco J, Moreno-Flores Adriana C, Vazquez-Pedroza Erick, Arreola-Guerra José M, González-Gámez Mario
Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico.
Laboratorio de Biotecnología, Departamento Ingeniería Bioquímica, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico.
Molecules. 2024 Dec 28;30(1):70. doi: 10.3390/molecules30010070.
Currently, a global health crisis is being caused by microbial resistance, in which plays a crucial role, being considered the highest-priority microorganism by the World Health Organization (WHO) for discovering new antibiotics. As a result, phytochemicals have emerged as a potential alternative to combat resistant strains, since they can exert antimicrobial activity through various mechanisms and, at the same time, represent a more natural and safe option. This study analyzes the antimicrobial effects of guava leaf extract in ten clinical isolates of extensively drug-resistant (XDR) , using the agar diffusion technique and the microdilution method to determine the minimum inhibitory concentrations (MICs). Additionally, possible improvements in antimicrobial activity after the purification of polyphenolic compounds and potential synergy with the antibiotic gentamicin are examined in this research. Moreover, the effect of the plant extract in cell line A549 derived from lung tissue was also evaluated. The extract exhibited antimicrobial activity against all the strains studied, and the purification of polyphenols along with the combination with gentamicin improved the extract activity. The presence of the plant extract induced morphological changes in the lung cells after 24 h of exposure. Therefore, L. leaf extract is a potential antimicrobial agent.
目前，微生物耐药性正在引发一场全球健康危机，在这场危机中起着关键作用，被世界卫生组织（WHO）视为发现新抗生素的最优先考虑的微生物。因此，植物化学物质已成为对抗耐药菌株的一种潜在替代品，因为它们可以通过多种机制发挥抗菌活性，同时代表着一种更天然、更安全的选择。本研究采用琼脂扩散技术和微量稀释法测定最低抑菌浓度（MICs），分析了番石榴叶提取物对10株广泛耐药（XDR）临床分离株的抗菌作用。此外，本研究还考察了多酚类化合物纯化后抗菌活性的可能提高以及与抗生素庆大霉素的潜在协同作用。此外，还评估了植物提取物对源自肺组织的A549细胞系的作用。提取物对所有研究菌株均表现出抗菌活性，多酚的纯化以及与庆大霉素的联合使用提高了提取物的活性。暴露24小时后，植物提取物的存在诱导了肺细胞的形态变化。因此，番石榴叶提取物是一种潜在的抗菌剂。
