Kotsias B A, Muchnik S, Arrizurieta E E, Losavio A S, Sosa M
Exp Neurol. 1985 Apr;88(1):56-67. doi: 10.1016/0014-4886(85)90113-x.
We studied the ionic and water content and the resting membrane potential of rat extensor digitorum longus EDL muscles at different times after unilateral nerve crush. Intracellular potassium concentration decreased progressively during the 1st week after nerve crush whereas intracellular sodium concentration increased significantly. At about day 10, when functional reinnervation (presence of end-plate potentials and miniature end-plate potentials) was detected, the above changes tended to return to control values. In addition, there was a significant difference between muscles with long and short nerve stumps. These results suggest a neurogenic dependency of muscle hydroelectrolytic composition. The decrease in resting membrane potential was greatest after 6.5 days of denervation when changes in the internal ionic concentration were maximum; however, these ionic changes contributed little to the decrease. The recovery of the resting membrane potential commenced at least 48 h before the first signs of functional reinnervation (10th day). This finding suggested an important contribution of some neurotrophic material in early stages of the reinnervation when nerve-muscle contacts were already established. Later, the contribution of mechanical activity to the restoration of the RMP became apparent (20th day); fibrillation potentials had disappeared by that time.
我们研究了单侧神经挤压后不同时间大鼠趾长伸肌（EDL）的离子和水分含量以及静息膜电位。神经挤压后的第1周内，细胞内钾浓度逐渐降低，而细胞内钠浓度显著升高。在大约第10天，当检测到功能性再支配（存在终板电位和微小终板电位）时，上述变化趋于恢复到对照值。此外，长神经残端和短神经残端的肌肉之间存在显著差异。这些结果表明肌肉水电解质组成存在神经源性依赖性。去神经支配6.5天后静息膜电位的降低最大，此时内部离子浓度变化最大；然而，这些离子变化对静息膜电位降低的贡献很小。静息膜电位的恢复至少在功能性再支配的第一个迹象（第10天）出现前48小时就开始了。这一发现表明，在神经肌肉接触已经建立的再支配早期，某些神经营养物质起了重要作用。后来，机械活动对静息膜电位恢复的贡献变得明显（第20天）；此时纤颤电位已经消失。
