Yang Qilin, Cui Wenyang, Guan Zijun, Wang Zhenzhen, Jahan Israt, Li Ping, Qin Feng, Qiao Xi, Liu Bo, Yan Jian
Key Laboratory of Agro-Environment in Tropics, Ministry of Agriculture and Rural Affairs, Guangdong Engineering Research Centre for Modern Eco-Agriculture and Circular Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Int J Mol Sci. 2024 Dec 25;26(1):64. doi: 10.3390/ijms26010064.
, one of the world's most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment-particularly the role of flavonoids in shaping soil microbial communities-remain underexplored. In this study, we identified and quantified ten flavonoids from root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin. To examine their impact, crude flavonoid extracts were applied to soil in potted experiments, which demonstrated that these compounds significantly increased soil fungal diversity and boosted the relative abundance of arbuscular mycorrhizal fungi (AMF). Furthermore, KEGG pathway analysis revealed that flavonoid addition elevated the copy numbers of genes involved in nitrogen cycling and metabolic functions, enhancing nutrient availability and microbial activity. Additionally, crude flavonoid extracts promoted the relative abundance of beneficial soil bacteria, such as Achromobacter, as well as AMF, both of which contribute to nutrient acquisition, plant growth, and soil health. These findings indicate that 's flavonoids can alter soil microbial community composition, thereby creating a favorable environment that reinforces its competitive edge over native plants.
作为世界上最具破坏性的入侵物种之一，以造成重大的生态和经济危害而闻名。虽然广泛的研究集中在其生长特性、次生代谢产物和控制措施上，但其与环境的化学相互作用，特别是黄酮类化合物在塑造土壤微生物群落中的作用，仍未得到充分探索。在本研究中，我们使用超高效液相色谱-质谱联用仪（UPLC-MS）从根际分泌物中鉴定并定量了十种黄酮类化合物，包括滨蓟黄素、异鼠李素和蜜橘黄素。为了研究它们的影响，在盆栽实验中将粗黄酮提取物施用于土壤，结果表明这些化合物显著增加了土壤真菌多样性，并提高了丛枝菌根真菌（AMF）的相对丰度。此外，京都基因与基因组百科全书（KEGG）通路分析表明，添加黄酮类化合物提高了参与氮循环和代谢功能的基因拷贝数，增强了养分有效性和微生物活性。此外，粗黄酮提取物促进了有益土壤细菌（如无色杆菌）以及AMF的相对丰度，这两者都有助于养分获取、植物生长和土壤健康。这些发现表明，[物种名称]的黄酮类化合物可以改变土壤微生物群落组成，从而创造一个有利的环境，增强其相对于本地植物的竞争优势。
