Guo Hong-Bo, Zhao Jia-Chen, Liu Wei-Ye, Bi Yan-Di, Sibirina Lidiya Alekseevna, Yu Xiao-Dan
College of Life Engineering, Shenyang Institute of Technology, Fushun, China.
Primorye State Agricultural Academy, Ussuriysk, Russia.
Sci Rep. 2025 Jun 2;15(1):19273. doi: 10.1038/s41598-025-03963-z.
Ectomycorrhizae (ECM) play a critical role in enhancing plant growth and health. However, the influence of artificially established ectomycorrhizal symbioses on the structure and function of rhizosphere microbial communities remains inadequately understood. In this study, a symbiotic relationship between Hebeloma hiemale and Quercus mongolica was established to investigate the influence of ECM on soil microbial communities in the rhizosphere of the host plant. High-throughput sequencing revealed that H. hiemale inoculation altered the evenness of both the fungal and bacterial communities and reduced the diversity of the bacterial community relative to the blank control. In particular, several bacterial genera with an enhanced capacity for nutrient cycling, contaminant degradation, and host plant protection were enriched following H. hiemale inoculation. Shifts in fungal community structure suggest potential benefits for the host plant, including reduced cadmium uptake, enhanced mercury remediation, and increased protection against pathogens. Our results highlight the complex interactions between ECM and rhizosphere microbial communities to enable a better understanding of the importance of multi-species relationships in plant-microbe symbioses and their ecological implications.
外生菌根（ECM）在促进植物生长和健康方面发挥着关键作用。然而，人工建立的外生菌根共生关系对根际微生物群落结构和功能的影响仍未得到充分了解。在本研究中，建立了毛柄环锈伞与蒙古栎之间的共生关系，以研究外生菌根对宿主植物根际土壤微生物群落的影响。高通量测序显示，接种毛柄环锈伞改变了真菌和细菌群落的均匀度，并且相对于空白对照降低了细菌群落的多样性。特别是，接种毛柄环锈伞后，几个具有增强的养分循环、污染物降解和宿主植物保护能力的细菌属得到了富集。真菌群落结构的变化表明对宿主植物有潜在益处，包括减少镉吸收、增强汞修复以及增强对病原体的防护。我们的结果突出了外生菌根与根际微生物群落之间的复杂相互作用，以便更好地理解多物种关系在植物-微生物共生中的重要性及其生态意义。
