Yan Kan, Lu Xin, Li Wandi, Sun Chao, Zhou Xueqiong, Wang Youyou
School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2025 Jan 2;26(1):323. doi: 10.3390/ijms26010323.
The Hypericaceae family, comprising nine genera and over seven hundred species, includes plants traditionally used for medicinal purposes. In this study, we performed high-throughput sequencing on three species: , , and , and conducted comparative genomic analyses with related species. The chloroplast genome sizes were 152,654 bp, 122,570 bp, and 137,652 bp, respectively, with an average GC content of 37.9%. All genomes showed a quadripartite structure, with significant variations in IR regions (3231-26,846 bp). The total number of genes ranged from 91 to 129. SSRs were predominantly located in the LSC region, with mononucleotide repeats being dominant. Comparative analysis identified several hotspot regions, including , , , and in the LSC region and , , , and in the SSC region. Nucleotide polymorphism analysis revealed eight highly variable regions and eleven gene loci, providing potential molecular markers for species identification. Phylogenetic analysis indicated that and are closely related to , with and being closest relatives and as their sister species. These findings provide molecular tools for species identification and insights for conservation strategies of medicinal species.
金丝桃科包含9个属和700多个物种，其中包括传统上用于药用的植物。在本研究中，我们对三种植物：[此处原文缺失三种植物的具体名称]进行了高通量测序，并与相关物种进行了比较基因组分析。叶绿体基因组大小分别为152,654 bp、122,570 bp和137,652 bp，平均GC含量为37.9%。所有基因组均呈现四分体结构，IR区域存在显著变异（3231 - 26,846 bp）。基因总数在91至129个之间。简单重复序列（SSRs）主要位于LSC区域，其中单核苷酸重复占主导。比较分析确定了几个热点区域，包括LSC区域的[此处原文缺失具体区域名称]以及SSC区域的[此处原文缺失具体区域名称]。核苷酸多态性分析揭示了八个高度可变区域和十一个基因位点，为物种鉴定提供了潜在的分子标记。系统发育分析表明[此处原文缺失具体物种名称]与[此处原文缺失具体物种名称]密切相关，[此处原文缺失具体物种名称]和[此处原文缺失具体物种名称]是近亲，[此处原文缺失具体物种名称]是它们的姊妹物种。这些发现为物种鉴定提供了分子工具，并为药用物种的保护策略提供了见解。
