腹部手术患者脂肪组织中膳食支链氨基酸和芳香族氨基酸与瘦素和FTO基因调控的关系。
The relationship between dietary branched-chain and aromatic amino acids with the regulation of leptin and FTO genes in adipose tissue of patients undergoing abdominal surgery.
作者信息
Teymoori Farshad, Farhadnejad Hossein, Norouzzadeh Mostafa, Jahromi Mitra Kazemi, Saber Niloufar, Mokhtari Ebrahim, Asghari Golaleh, Yuzbashian Emad, Mirmiran Parvin, Khalaj Alireza, Zarkesh Maryam, Hedayati Mehdi, Vafa Mohammadreza
机构信息
Nutritional Sciences Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Amino Acids. 2025 Jan 11;57(1):8. doi: 10.1007/s00726-024-03441-2.
Recent studies have suggested that the interaction between diet and an individual's genetic predisposition can determine the likelihood of obesity and various metabolic disorders. The current study aimed to examine the association of dietary branched-chain amino acids(BCAAs) and aromatic amino acids(AAAs) with the expression of the leptin and FTO genes in the visceral and subcutaneous adipose tissues of individuals undergoing surgery. This cross-sectional study was conducted on 136 Iranian adults, both men and women, aged ≥18 years. The samples were selected from patients admitted for abdominal surgeries. The dietary intake of BCAAs and AAAs was determined using a valid and reliable 168-item food frequency questionnaire. Using the quantitative PCR method, leptin and FTO mRNA expression was measured in both visceral and subcutaneous fat tissues. The mean age of the participants was 39.8 ± 12.7 years, and the mean intake of BCAAs and AAAs was 17.7 ± 0.9 and 9.3 ± 0.3% of protein per day, respectively. In overweight-obese patients(body mass index = 25-34.9 kg/m), the intake of BCAAs(β:-0.75,95%CI:-1.47,-0.03), valine(β:-0.78,95%CI:-1.51,-0.05), and tyrosine(β:-0.81,95%CI:-1.55,-0.06) was inversely associated with FTO gene expression in subcutaneous fat tissue in adjusted model. In morbidly obese patients(body mass index ≥ 35 kg/m), a higher intake of total BCAAs(β:1.10,95%CI:0.07-2.13), leucine(β:1.07,95%CI:0.03-2.13), and isoleucine(β:1.49,95%CI:0.46-2.52) was associated with an increase of leptin gene expression in subcutaneous fat tissue. Our findings suggest that dietary BCAA may associated with gene expression in adipose tissues, potentially influencing obesity-related metabolic pathways. Further prospective studies are warranted to validate results and elucidate the potential for dietary interventions targeting amino acids intake in obesity management.
近期研究表明，饮食与个体遗传易感性之间的相互作用可决定肥胖及各种代谢紊乱的可能性。本研究旨在探讨饮食中的支链氨基酸（BCAAs）和芳香族氨基酸（AAAs）与接受手术个体的内脏和皮下脂肪组织中瘦素和FTO基因表达之间的关联。这项横断面研究针对136名年龄≥18岁的伊朗成年男女开展。样本选自因腹部手术入院的患者。使用一份有效且可靠的包含168个项目的食物频率问卷来确定BCAAs和AAAs的饮食摄入量。采用定量PCR方法，测量内脏和皮下脂肪组织中瘦素和FTO mRNA的表达。参与者的平均年龄为39.8±12.7岁，BCAAs和AAAs的平均摄入量分别为每日蛋白质的17.7±0.9%和9.3±0.3%。在超重肥胖患者（体重指数=25-34.9kg/m）中，调整模型下，皮下脂肪组织中BCAAs（β：-0.75，95%CI：-1.47，-0.03）、缬氨酸（β：-0.78，95%CI：-1.51，-0.05）和酪氨酸（β：-0.81，95%CI：-1.55，-0.06）的摄入量与FTO基因表达呈负相关。在病态肥胖患者（体重指数≥35kg/m）中，皮下脂肪组织中较高的总BCAAs（β：1.10，95%CI：0.07-2.13）、亮氨酸（β：1.07，95%CI：0.03-2.13）和异亮氨酸（β：1.49，95%CI：0.46-2.52）摄入量与瘦素基因表达增加相关。我们的研究结果表明，饮食中的BCAA可能与脂肪组织中的基因表达相关，潜在影响肥胖相关的代谢途径。有必要开展进一步的前瞻性研究以验证结果，并阐明针对肥胖管理中氨基酸摄入的饮食干预潜力。
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