Meng Ziyi, Yan Xu, Azarakhsh Nima, Duan Hanying, Ramaswamy Hosahalli S, Wang Chao
Department of Food Science and Technology, Jinan University, Guangzhou 510632, China; China Mengniu Dairy Co., Ltd, Hohhot 011517, China.
Department of Food Science and Technology, Jinan University, Guangzhou 510632, China.
Food Chem. 2025 Apr 15;471:142792. doi: 10.1016/j.foodchem.2025.142792. Epub 2025 Jan 5.
As an essential B vitamin, folate participates in one‑carbon metabolism. The 5-methyltetrahydrofolate (5-MTHF) avoids the drawbacks associated with folic acid and native folylpolyglutamate folate in food, thereby emerging as a superior alternative to folate supplement. To enhance the stability and digestibility of 5-MTHF, nanoliposome (NL) was modified using a layer-by-layer self-assembly method with chitosan (CH) and pectin (P). Chitosan-nanoliposome (CH-NL) and pectin-chitosan-nanoliposome (P-CH-NL) were created, each featuring a core-shell structure. P-CH-NL achieved an encapsulation efficiency of 64.62 %, loading efficiency of 1.05 mg/g, and particle size of 285.86 nm. It exhibited better physical stability and 5-MTHF retention (>80 %) under various conditions, including salt and pH variations, as well as oxidative, thermal, fermentation, and UV stress. During in vitro digestion, P-CH-NL protected 5-MTHF until it was released into the small intestine. This study highlighted the application prospects of multilayer liposome-loaded 5-MTHF as a stable, highly digestible folate supplement.
作为一种必需的B族维生素，叶酸参与一碳代谢。5-甲基四氢叶酸（5-MTHF）避免了与叶酸以及食物中的天然聚谷氨酸叶酸相关的缺点，从而成为叶酸补充剂的优质替代品。为提高5-MTHF的稳定性和消化率，采用壳聚糖（CH）和果胶（P）通过层层自组装法对纳米脂质体（NL）进行修饰。制备了壳聚糖-纳米脂质体（CH-NL）和果胶-壳聚糖-纳米脂质体（P-CH-NL），二者均具有核壳结构。P-CH-NL的包封率为64.62%，载药量为1.05 mg/g，粒径为285.86 nm。在盐、pH值变化以及氧化、热、发酵和紫外线应激等各种条件下，它表现出更好的物理稳定性和5-MTHF保留率（>80%）。在体外消化过程中，P-CH-NL可保护5-MTHF，直至其释放到小肠中。本研究突出了多层脂质体负载5-MTHF作为一种稳定、高消化率叶酸补充剂的应用前景。
