Yuan Yufeng, Wu Jie, Wei Fanqi, Wan Zhangmin, Dong Yintian, Lu Yi, Yang Pu, Jin Yongcan, Saddler Jack
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, and Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; Forest Product Biotechnology/Bioenergy Group, Department of Wood Science, University of British Columbia, 2424 Main Mal, Vancouver V6T 1Z4, Canada.
Forest Product Biotechnology/Bioenergy Group, Department of Wood Science, University of British Columbia, 2424 Main Mal, Vancouver V6T 1Z4, Canada; Advanced Renewable Materials Lab, Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver V6T 1N4, Canada.
Int J Biol Macromol. 2025 Mar;296:139674. doi: 10.1016/j.ijbiomac.2025.139674. Epub 2025 Jan 9.
Modern enzyme cocktails often include lytic polysaccharide monooxygenase (LPMO) as an accessory enzyme that enhances cellulose accessibility during hydrolysis. Although lignin is known to generally impede cellulose hydrolysis, previous research has demonstrated lignin's potential to act as a co-factor in boosting LPMO activity and that the negative impact of lignin limiting enzyme accessibility can be mitigated by sulfonated. When sulphonated lignin was added to microcrystalline cellulose (Avicel) the activity of the lytic polysaccharide monooxygenase (LPMO) was boosted, as determined when using a quartz crystal microbalance and dissipation monitoring (QCM-D). Further assessment via scanning electron microscopy, Simon's staining and nitrogen adsorption indicated that the addition of sulphonated lignin with the LPMO also increased cellulose accessibility.
现代酶混合物通常包含裂解多糖单加氧酶（LPMO）作为辅助酶，该酶可在水解过程中提高纤维素的可及性。尽管已知木质素通常会阻碍纤维素水解，但先前的研究表明，木质素具有作为辅助因子提高LPMO活性的潜力，并且木质素限制酶可及性的负面影响可通过磺化来减轻。当将磺化木质素添加到微晶纤维素（微晶纤维素）中时，如使用石英晶体微天平及耗散监测（QCM-D）所测定的那样，裂解多糖单加氧酶（LPMO）的活性得到了提高。通过扫描电子显微镜、西蒙染色和氮吸附进行的进一步评估表明，将磺化木质素与LPMO一起添加也增加了纤维素的可及性。
