The bHLH transcription factor DlbHLH68 positively regulates DlSPS1 expression to promote sucrose biosynthesis in longan.

Sucrose is an important factor affecting plant growth and fruit quality, but the molecular regulatory mechanism of sucrose biosynthesis in longan is not yet understood. Here, we characterized a transcription factor, DlbHLH68, positively regulates sucrose accumulation in longan. Subcellular localization and transcriptional activity analysis indicated that DlbHLH68 is a nuclear transcriptional activator. Overexpressing DlbHLH68 in Arabidopsis enhanced sucrose content, plant height, and the relative expression level of sucrose phosphate synthase genes (AtSPS1 and AtSPS2). Yeast one-hybrid and dual-luciferase reporter assays indicated that DlbHLH68 was able to activate the expression of DlSPS1, the homology gene of AtSPS1. As expected, overexpression of DlSPS1 significantly increased the sucrose content in transgenic Arabidopsis and longan fruits. Collectively, this study reveals that DlbHLH68 is a positive regulator in sucrose accumulation by activating DlSPS1 expression to mediate sucrose biosynthesis, which is helpful for understanding the molecular basis of sucrose biosynthesis and accumulation in longan fruit and provides candidate genes for further breeding.