Tyagi Akanksha, Choi Yu-Yeong, Shan Lingyue, Vinothkanna Annadurai, Lee Eun-Seok, Chelliah Ramachandran, Barathikannan Kaliyan, Raman Sivakumar Thasma, Park Se Jin, Jia Ai-Qun, Choi Geun Pyo, Oh Deog Hwan
Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, Purdue, IN, USA.
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, South Korea.
NPJ Sci Food. 2025 Jan 11;9(1):5. doi: 10.1038/s41538-025-00369-z.
Chronic stress disrupts gut microbiota homeostasis, contributing to anxiety and depression. This study explored the effects of Limosilactobacillus reuteri fermented brown rice (FBR) on anxiety using an ICR mouse chronic mild stress (CMS) model. Anxiety was assessed through body weight, corticosterone levels, neurotransmitter profiles, and behavioral tests. A four-week FBR regimen reduced corticosterone, restored neurotransmitters like gamma-aminobutyric acid (GABA) and serotonin, and improved anxiety-related behaviors. Metagenomic (16S rRNA) and metabolomic analyses revealed enhanced amino acid metabolism, energy metabolism, and short-chain fatty acid (SCFA) production in FBR-treated mice. FBR-enriched beneficial gut bacteria, aligning the microbiota profile with that of non-stressed mice. FBR also modulated GABA receptor-related gene expression, promoting relaxation. Network pharmacology identified quercetin, GABA, glutamic acid, phenylalanine, and ferulic acid as bioactive compounds with neuroprotective potential. These findings highlight FBR's potential as a gut-brain axis-targeted therapeutic for anxiety and stress-related disorders.
慢性应激会破坏肠道微生物群的稳态，导致焦虑和抑郁。本研究使用ICR小鼠慢性轻度应激（CMS）模型探讨了罗伊氏乳杆菌发酵糙米（FBR）对焦虑的影响。通过体重、皮质酮水平、神经递质谱和行为测试来评估焦虑。为期四周的FBR方案降低了皮质酮水平，恢复了γ-氨基丁酸（GABA）和血清素等神经递质，并改善了与焦虑相关的行为。宏基因组（16S rRNA）和代谢组学分析显示，FBR处理的小鼠的氨基酸代谢、能量代谢和短链脂肪酸（SCFA）生成增强。FBR使有益肠道细菌富集，使微生物群谱与非应激小鼠的谱一致。FBR还调节了GABA受体相关基因的表达，促进了放松。网络药理学确定槲皮素、GABA、谷氨酸、苯丙氨酸和阿魏酸为具有神经保护潜力的生物活性化合物。这些发现凸显了FBR作为一种针对肠-脑轴治疗焦虑和应激相关疾病的潜力。
