Wu Chongyun, Li Meng, Chen Zhe, Feng Shu, Deng Qianting, Duan Rui, Liu Timon Cheng-Yi, Yang Luodan
Laboratory of Exercise and Neurobiology, School of Physical Education and Sports Science, South China Normal University, Guangzhou, 510006, Guangdong, China.
Laboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal University, Guangzhou, 510006, China.
Transl Psychiatry. 2025 Jan 11;15(1):8. doi: 10.1038/s41398-025-03228-3.
Repeated closed-head injuries (rCHI) from activities like contact sports, falls, military combat, and traffic accidents pose a serious risk due to their cumulative impact on the brain. Often, rCHI is not diagnosed until symptoms of irreversible brain damage appear, highlighting the need for preventive measures. This study assessed the prophylactic efficacy of remote photobiomodulation (PBM) targeted at the lungs against rCHI-induced brain injury and associated behavioral deficits. Utilizing the "Marmarou" weight-drop model, rCHI was induced in rats on days 0, 5, and 10. Remote PBM, employing an 808 nm continuous wave laser, was administered daily in 2-min sessions per lung side over 20 days. Behavioral deficits were assessed through three-chamber social interaction, forced swim, grip strength, open field, elevated plus maze, and Barnes maze tests. Immunofluorescence staining and 3D reconstruction evaluated neuronal damage, apoptosis, degeneration, and the morphology of microglia and astrocytes, as well as astrocyte and microglia-mediated excessive synapse elimination. Additionally, 16S rDNA amplicon sequencing analyzed changes in the lung microbiome following remote PBM treatment. Results demonstrated that remote PBM significantly improved depressive-like behaviors, motor dysfunction, and social interaction impairment while enhancing grip strength and reducing neuronal damage, apoptosis, and degeneration induced by rCHI. Analysis of lung microbiome changes revealed an enrichment of lipopolysaccharide (LPS) biosynthesis pathways, suggesting a potential link to neuroprotection. Furthermore, remote PBM mitigated hyperactivation of cortical microglia and astrocytes and significantly reduced excessive synaptic phagocytosis by these cells, highlighting its potential as a preventive strategy for rCHI with neuroprotective effects.
来自诸如接触性运动、跌倒、军事战斗和交通事故等活动的反复闭合性头部损伤（rCHI），因其对大脑的累积影响而构成严重风险。通常，rCHI直到出现不可逆脑损伤的症状才被诊断出来，这凸显了预防措施的必要性。本研究评估了针对肺部的远程光生物调节（PBM）对rCHI诱导的脑损伤及相关行为缺陷的预防效果。利用“Marmarou”重物坠落模型，在第0天、第5天和第10天对大鼠诱导rCHI。采用808 nm连续波激光进行远程PBM，在20天内每天对每侧肺进行2分钟的照射。通过三室社交互动、强迫游泳、握力、旷场、高架十字迷宫和巴恩斯迷宫测试评估行为缺陷。免疫荧光染色和三维重建评估神经元损伤、细胞凋亡、变性以及小胶质细胞和星形胶质细胞的形态，以及星形胶质细胞和小胶质细胞介导的过度突触消除。此外，16S rDNA扩增子测序分析了远程PBM治疗后肺部微生物群的变化。结果表明，远程PBM显著改善了抑郁样行为、运动功能障碍和社交互动障碍，同时增强了握力，减少了rCHI诱导的神经元损伤、细胞凋亡和变性。对肺部微生物群变化的分析显示脂多糖（LPS）生物合成途径富集，提示其与神经保护存在潜在联系。此外，远程PBM减轻了皮质小胶质细胞和星形胶质细胞的过度激活，并显著减少了这些细胞的过度突触吞噬，凸显了其作为具有神经保护作用的rCHI预防策略的潜力。
