Ferencík M, Bergendi L, Mandel L, Kovárů F, Stefanovic J
Folia Microbiol (Praha). 1985;30(1):65-75. doi: 10.1007/BF02922500.
The activities of lysozyme, myeloperoxidase, and elastase were lower in PMNs and AMs from GF and AF Minnesota miniature piglets than in the leukocytes from their CONV counterparts. In the spleen and serum of gnotobiotic piglets only the levels of lysozyme were slightly reduced. Substantially depressed activities of these LEs were found also in PMNs from precolostral piglets in comparison with PMNs from their CONV mother. The bisassociation of GF piglets with Enterococcus liquefaciens and Escherichia coli caused an increase of LE activities in their AMs, spleens, and sera. Fewer LEs were released after phygocytic stimulation with zymosan from PMNs of GF, AF, and precolostral piglets than from PMNs of CONV animals of the same age. These data suggest that the antigenic-microbial stimulation is important for the development of normal lysosomal enzyme activities in PMNs and AMs from gnotobiotic animals.
无菌（GF）和无特定病原体（AF）明尼苏达小型仔猪的中性粒细胞（PMN）和肺泡巨噬细胞（AM）中，溶菌酶、髓过氧化物酶和弹性蛋白酶的活性低于其对照（CONV）仔猪的白细胞。在无菌仔猪的脾脏和血清中，只有溶菌酶水平略有降低。与来自对照母猪的PMN相比，初乳前仔猪的PMN中这些溶酶体酶（LE）的活性也显著降低。GF仔猪与液化肠球菌和大肠杆菌双联合后，其AM、脾脏和血清中的LE活性增加。与同龄对照动物的PMN相比，用酵母聚糖对GF、AF和初乳前仔猪的PMN进行吞噬刺激后释放的LE较少。这些数据表明，抗原-微生物刺激对于无菌动物PMN和AM中正常溶酶体酶活性的发展很重要。
