A Sakran Nawar, Cherif Slim, Shawqi Algburi Firas
Biology Department, College of Science, Sfax University, Tunisia.
Biochemistry Department, College of Medicine, Tikrit University, Tikrit, Iraq.
Cell Mol Biol (Noisy-le-grand). 2025 Jan 12;70(12):110-115. doi: 10.14715/cmb/2024.70.12.15.
Chronic kidney disease (CKD) is often complicated by diabetes, impacting various biochemical and immunological markers. This study aimed to investigate the relationship between irisin, apelin-13, and immunological markers IL-1α and IL-1β in diabetic patients with CKD. This cross-sectional study was conducted from January to June 2023 in a tertiary care hospital in Tikrit City, Iraq. This study included 120 CKD patients and a control group including 20 healthy individuals. Patients were included in the study by convenience sampling method. Participants were evaluated using ELISA kits for irisin, apelin-13, and cytokines, with blood samples analyzed for relevant biochemical markers. Patients had irisin levels of 10.98 ± 2.5 ng/mL, significantly different from non-diabetic patients (12.40 ± 3.54 ng/mL) and controls (5.36 ± 1.06 ng/mL) (p<0.001). Apelin-13 was higher in diabetic patients (537.71 ± 124.78 pg/mL) compared to controls (181.26 ± 29.98 pg/mL) (p<0.001). IL-1α levels in diabetic patients were 715.30 ± 392.48 pg/mL, significantly higher than in control patients (206.27 ± 26.49 pg/mL) (p<0.001). IL-1β levels were 351.50 ± 81.82 pg/mL in diabetics, also higher than in control (145.79 ± 38.49 pg/mL) (p<0.001). The study highlights significant associations between biochemical markers and CKD in diabetic patients. Elevated levels of irisin, apelin-13, IL-1α, and IL-1β may serve as potential biomarkers for diabetes-related CKD complications.
慢性肾脏病（CKD）常并发糖尿病，影响多种生化和免疫指标。本研究旨在探讨鸢尾素、Apelin-13与慢性肾脏病糖尿病患者免疫指标白细胞介素-1α（IL-1α）和白细胞介素-1β（IL-1β）之间的关系。本横断面研究于2023年1月至6月在伊拉克提克里特市的一家三级护理医院进行。本研究纳入了120例慢性肾脏病患者和一个包含20名健康个体的对照组。采用便利抽样法将患者纳入研究。使用酶联免疫吸附测定（ELISA）试剂盒对参与者的鸢尾素、Apelin-13和细胞因子进行评估，并对血样进行相关生化指标分析。患者的鸢尾素水平为10.98±2.5纳克/毫升，与非糖尿病患者（12.40±3.54纳克/毫升）和对照组（5.36±1.06纳克/毫升）有显著差异（p<0.001）。与对照组（181.26±29.98皮克/毫升）相比，糖尿病患者的Apelin-13水平更高（537.71±124.78皮克/毫升）（p<0.001）。糖尿病患者的IL-1α水平为715.30±392.48皮克/毫升，显著高于对照患者（206.27±26.49皮克/毫升）（p<0.001）。糖尿病患者的IL-1β水平为351.50±81.82皮克/毫升，也高于对照组（145.79±38.49皮克/毫升）（p<0.001）。该研究突出了糖尿病患者生化指标与慢性肾脏病之间的显著关联。鸢尾素、Apelin-13、IL-1α和IL-1β水平升高可能作为糖尿病相关慢性肾脏病并发症的潜在生物标志物。
