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用于测量人类细胞中葡萄糖和乳酸代谢变化的活细胞代谢分析仪方案

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells.

作者信息

Miki Kenji, Yagi Mikako, Igami Ko, Kittaka Hiroki, Tani Akito, Horiuchi Natsuki, Fukumoto Satoshi, Yoshimoto Koji, Uchiumi Takeshi

机构信息

Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan; Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.

Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103518. doi: 10.1016/j.xpro.2024.103518. Epub 2025 Jan 10.

DOI:10.1016/j.xpro.2024.103518
PMID:39799573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11772134/
Abstract

Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

摘要

了解与糖酵解依赖性相关的代谢状况对于开发癌症和再生医学的新疗法至关重要。本方案详细介绍了一种使用活细胞代谢分析仪（LiCellMo）来测量培养的人类细胞中葡萄糖消耗和乳酸生成的连续变化的方法。LiCellMo提供连续代谢变化的实时数据，改善了在包括癌症和再生治疗在内的各种情况下对这些过程的测量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/044fc9fb3652/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/9003ddda7167/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/b5707edbf56c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/23db2e6783b7/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/a787d8715c7c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/09e7c2fb0511/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/41b6a14d9802/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/cbde1b34c526/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/18847f55c429/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/ada4c82a7d2e/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/044fc9fb3652/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/9003ddda7167/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/b5707edbf56c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/23db2e6783b7/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/a787d8715c7c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/09e7c2fb0511/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/41b6a14d9802/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/cbde1b34c526/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/18847f55c429/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/ada4c82a7d2e/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b456/11772134/044fc9fb3652/gr9.jpg

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