利用磁共振波谱研究自噬过程中的糖酵解代谢
Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.
作者信息
Chung Y-L, Leach M O, Eykyn T R
机构信息
Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, London, United Kingdom.
Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, London, United Kingdom.
出版信息
Methods Enzymol. 2017;588:133-153. doi: 10.1016/bs.mie.2016.09.078. Epub 2016 Nov 17.
Abstract
Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells and tissues, how to measure the rate of steady-state lactate excretion and glucose uptake by H-MRS in autophagic cells, and details of the real-time measurement of [1-C] pyruvate to lactate exchange in autophagic cells by C-MRS-DNP (dynamic nuclear polarization).
摘要
研究发现，经历饥饿和治疗诱导自噬的癌细胞表现出细胞内乳酸减少、稳态乳酸排泄率降低以及实时丙酮酸-乳酸交换率降低，这表明自噬细胞中的糖酵解代谢发生了改变。在本章中，我们描述了使用氢磁共振波谱（MRS）测量自噬细胞和组织中内源性细胞乳酸和葡萄糖浓度的技术细节，如何通过氢MRS测量自噬细胞中稳态乳酸排泄率和葡萄糖摄取率，以及通过碳MRS-动态核极化（C-MRS-DNP）实时测量自噬细胞中[1-碳]丙酮酸向乳酸交换的细节。
相似文献
1
Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy.利用磁共振波谱研究自噬过程中的糖酵解代谢
Methods Enzymol. 2017;588:133-153. doi: 10.1016/bs.mie.2016.09.078. Epub 2016 Nov 17.
2
Reduced Warburg effect in cancer cells undergoing autophagy: steady- state 1H-MRS and real-time hyperpolarized 13C-MRS studies.自噬癌细胞中瓦伯格效应的降低：稳态¹H-MRS和实时超极化¹³C-MRS研究
PLoS One. 2014 Mar 25;9(3):e92645. doi: 10.1371/journal.pone.0092645. eCollection 2014.
3
Molecular detection of inflammation in cell models using hyperpolarized C-pyruvate.使用超极化 C-丙酮酸检测细胞模型中的炎症反应。
Theranostics. 2018 May 23;8(12):3400-3407. doi: 10.7150/thno.24322. eCollection 2018.
4
Combined hyperpolarized C-pyruvate MRS and F-FDG PET (hyperPET) estimates of glycolysis in canine cancer patients.联合极化 C-丙酮酸 MRS 和 F-FDG PET（hyperPET）评估犬癌症患者的糖酵解。
Eur J Radiol. 2018 Jun;103:6-12. doi: 10.1016/j.ejrad.2018.02.028. Epub 2018 Feb 25.
5
Hyperpolarized [1-C]pyruvate-to-[1-C]lactate conversion is rate-limited by monocarboxylate transporter-1 in the plasma membrane.在细胞膜中，[1-C]丙酮酸到[1-C]乳酸的转化受单羧酸转运蛋白-1 的限制。
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22378-22389. doi: 10.1073/pnas.2003537117. Epub 2020 Aug 24.
6
Using [1-(13) C]lactic acid for hyperpolarized (13) C MR cardiac studies.使用[1-(13)C]乳酸进行超极化(13)C磁共振心脏研究。
Magn Reson Med. 2015 Jun;73(6):2087-93. doi: 10.1002/mrm.25354. Epub 2014 Jul 9.
7
Probing human sperm metabolism using 13C-magnetic resonance spectroscopy.采用 13C 磁共振波谱技术探究人类精子代谢。
Mol Hum Reprod. 2019 Jan 1;25(1):30-41. doi: 10.1093/molehr/gay046.
8
In vivo measurement of normal rat intracellular pyruvate and lactate levels after injection of hyperpolarized [1-(13)C]alanine.注射高极化 [1-(13)C]丙氨酸后正常大鼠细胞内丙酮酸和乳酸水平的体内测量。
Magn Reson Imaging. 2011 Oct;29(8):1035-40. doi: 10.1016/j.mri.2011.07.001. Epub 2011 Aug 19.
9
Hyperpolarized [1-13C] pyruvate MR spectroscopy detect altered glycolysis in the brain of a cognitively impaired mouse model fed high-fat diet.高极化 [1-13C] 丙酮酸磁共振波谱检测高脂饮食喂养的认知障碍小鼠模型脑内糖酵解改变。
Mol Brain. 2018 Dec 18;11(1):74. doi: 10.1186/s13041-018-0415-2.
10
Assessment of mouse liver [1-13C]pyruvate metabolism by dynamic hyperpolarized MRS.动态极化 [1-13C]丙酮酸磁共振波谱评估小鼠肝脏代谢。
J Endocrinol. 2019 Sep 1;242(3):251-260. doi: 10.1530/JOE-19-0159.
引用本文的文献
1
Primary Metabolite Screening Shows Significant Differences between Embryogenic and Non-Embryogenic Callus of Tamarillo ( Cav.).初级代谢物筛选显示番茄树（Cav.）胚性愈伤组织和非胚性愈伤组织之间存在显著差异。
Plants (Basel). 2023 Aug 4;12(15):2869. doi: 10.3390/plants12152869.
2
Characterization of hepatic fatty acids using magnetic resonance spectroscopy for the assessment of treatment response to metformin in an eNOS mouse model of metabolic nonalcoholic fatty liver disease/nonalcoholic steatohepatitis.采用磁共振波谱技术对肝脏脂肪酸进行特征分析，以评估内皮型一氧化氮合酶（eNOS）小鼠代谢性非酒精性脂肪性肝病/非酒精性脂肪性肝炎模型中二甲双胍治疗反应。
NMR Biomed. 2023 Aug;36(8):e4932. doi: 10.1002/nbm.4932. Epub 2023 Apr 11.
3
Fasting increases susceptibility to acute myocardial ischaemia/reperfusion injury through a sirtuin-3 mediated increase in fatty acid oxidation.禁食通过 SIRT3 介导的脂肪酸氧化增加增加急性心肌缺血/再灌注损伤的易感性。
Sci Rep. 2022 Nov 29;12(1):20551. doi: 10.1038/s41598-022-23847-w.
4
Impact of reduced uterine perfusion pressure model of preeclampsia on metabolism of placenta, maternal and fetal hearts.子痫前期低子宫灌注压模型对胎盘、母胎心脏代谢的影响。
Sci Rep. 2022 Jan 21;12(1):1111. doi: 10.1038/s41598-022-05120-2.
5
Intracellular sodium elevation reprograms cardiac metabolism.细胞内钠离子浓度升高会重新编程心脏代谢。
Nat Commun. 2020 Aug 28;11(1):4337. doi: 10.1038/s41467-020-18160-x.
6
Age-Dependent Decline in Cardiac Function in Guanidinoacetate--Methyltransferase Knockout Mice.胍基乙酸 - 甲基转移酶基因敲除小鼠心脏功能的年龄依赖性衰退
Front Physiol. 2020 Jan 21;10:1535. doi: 10.3389/fphys.2019.01535. eCollection 2019.
7
Cardiac metabolomic profile of the naked mole-rat-glycogen to the rescue.裸鼹鼠的心脏代谢组学特征——糖原来救援。
Biol Lett. 2019 Nov 29;15(11):20190710. doi: 10.1098/rsbl.2019.0710. Epub 2019 Nov 27.
8
Evaluation of the combination of the dual m-TORC1/2 inhibitor vistusertib (AZD2014) and paclitaxel in ovarian cancer models.在卵巢癌模型中评估双重m-TORC1/2抑制剂维思他瑞（AZD2014）与紫杉醇联合使用的效果。
Oncotarget. 2017 Dec 6;8(69):113874-113884. doi: 10.18632/oncotarget.23022. eCollection 2017 Dec 26.
本文引用的文献
1
A comparison of quantitative methods for clinical imaging with hyperpolarized (13)C-pyruvate.超极化（13）C-丙酮酸临床成像定量方法的比较
NMR Biomed. 2016 Apr;29(4):387-99. doi: 10.1002/nbm.3468. Epub 2016 Jan 18.
2
Studies of Metabolism Using (13)C MRS of Hyperpolarized Probes.使用超极化探针的¹³C磁共振波谱法进行的代谢研究。
Methods Enzymol. 2015;561:1-71. doi: 10.1016/bs.mie.2015.04.001. Epub 2015 Jun 19.
3
A roadmap for interpreting (13)C metabolite labeling patterns from cells.解读细胞中（13）C代谢物标记模式的路线图。
Curr Opin Biotechnol. 2015 Aug;34:189-201. doi: 10.1016/j.copbio.2015.02.003. Epub 2015 Feb 28.
4
Hyperpolarized magnetic resonance as a sensitive detector of metabolic function.超极化磁共振作为代谢功能的灵敏探测器。
Biochemistry. 2014 Dec 2;53(47):7333-57. doi: 10.1021/bi501225t. Epub 2014 Nov 18.
5
Dichloroacetate induces autophagy in colorectal cancer cells and tumours.二氯乙酸诱导结直肠癌细胞和肿瘤发生自噬。
Br J Cancer. 2014 Jul 15;111(2):375-85. doi: 10.1038/bjc.2014.281. Epub 2014 Jun 3.
6
Reduced Warburg effect in cancer cells undergoing autophagy: steady- state 1H-MRS and real-time hyperpolarized 13C-MRS studies.自噬癌细胞中瓦伯格效应的降低：稳态¹H-MRS和实时超极化¹³C-MRS研究
PLoS One. 2014 Mar 25;9(3):e92645. doi: 10.1371/journal.pone.0092645. eCollection 2014.
7
Chemistry and biochemistry of 13C hyperpolarized magnetic resonance using dynamic nuclear polarization.使用动态核极化的 13C 超极化磁共振的化学和生物化学。
Chem Soc Rev. 2014 Mar 7;43(5):1627-59. doi: 10.1039/c3cs60124b. Epub 2013 Dec 20.
8
Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes.使用极化葡萄糖进行非侵入性的细胞质游离[NAD+]/[NADH] 比率的细胞内测定显示代谢表型存在很大差异。
J Biol Chem. 2014 Jan 24;289(4):2344-52. doi: 10.1074/jbc.M113.498626. Epub 2013 Dec 3.
9
Model free approach to kinetic analysis of real-time hyperpolarized 13C magnetic resonance spectroscopy data.无模型方法分析实时动态极化 13C 磁共振波谱数据的动力学。
PLoS One. 2013 Sep 4;8(9):e71996. doi: 10.1371/journal.pone.0071996. eCollection 2013.
10
Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research.通过成像技术分析癌症代谢：向临床研究转化的前景。
Neoplasia. 2011 Feb;13(2):81-97. doi: 10.1593/neo.101102.
Zotero 插件