• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种基于铁蛋白的Eg95纳米颗粒疫苗，佐以pCpG，在小鼠模型中引发针对囊型包虫病的强烈免疫反应。

A Ferritin-Based Eg95 Nanoparticle Vaccine Adjuvanted with pCpG Eliciting Robust Immune Responses Against Cystic Echinococcosis in Mice Model.

作者信息

Gao Xintao, Zhu Xizhou, Liu Xingjian, Zhou Chenghao, Shang Yuting, Wu Tong, Jia Hong, Zhang Zhifang, Li Yinü, Xin Ting

机构信息

National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

Bioproducts Engineering Center, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

出版信息

Int J Nanomedicine. 2025 Jan 8;20:309-325. doi: 10.2147/IJN.S499938. eCollection 2025.

DOI:10.2147/IJN.S499938
PMID:39802377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11725278/
Abstract

INTRODUCTION

Cystic echinococcosis (CE), a chronic disabling parasitic zoonosis, poses a great threat to public health and livestock production and causes huge economic losses globally. The commercial Quil-A-adjuvanted Eg95 vaccine was empirically effective for CE control; however, it is expensive and has side effects and insufficient immunity.

PURPOSE

This study aimed to employ a novel adjuvant consisting of a delivery system and an immune potentiator and assess its adjuvanticity to Eg95 antigen, thereby developing a safe and cost-effective novel vaccine against the disease.

METHODS

A ferritin-based Eg95 nanoparticle antigen was prepared and then mixed with a plasmid containing the TLR9 agonist CpG to formulate a novel nanovaccine. The safety and efficacy of the vaccine were evaluated in vitro and in vivo.

RESULTS

The nanovaccine induced potent and enduring Eg95-specific humoral and cellular immune responses, as well as protective immunity-associated Th1 polarization supported by the higher ratios of IgG2a/IgG1 and IFN-γ/IL-4. Meanwhile, this nanovaccines exhibited favorable safety and economic profiles.

CONCLUSION

Our data demonstrated that the ferritin-CpG hybrid is a promising combination adjuvant to upgrade the traditional Quil-A and this combination adjuvant-based nanovaccine presents good potential as an alternative to the commercial one for practical CE control.

摘要

引言

囊型包虫病（CE）是一种慢性致残性寄生虫人畜共患病，对公共卫生和畜牧业生产构成巨大威胁，并在全球范围内造成巨大经济损失。市售的Quil-A佐剂Eg95疫苗在控制CE方面经验证有效；然而，它价格昂贵，有副作用且免疫效果不足。

目的

本研究旨在采用一种由递送系统和免疫增强剂组成的新型佐剂，并评估其对Eg95抗原的佐剂活性，从而开发一种安全且经济高效的针对该疾病的新型疫苗。

方法

制备基于铁蛋白的Eg95纳米颗粒抗原，然后与含有Toll样受体9（TLR9）激动剂CpG的质粒混合，以制备新型纳米疫苗。在体外和体内评估该疫苗的安全性和有效性。

结果

该纳米疫苗诱导了强效且持久的Eg95特异性体液免疫和细胞免疫反应，以及由较高的IgG2a/IgG1和干扰素-γ（IFN-γ）/白细胞介素-4（IL-4）比值所支持的与保护性免疫相关的Th1极化。同时，这种纳米疫苗表现出良好的安全性和经济性。

结论

我们的数据表明，铁蛋白-CpG复合物是一种有前景的复合佐剂，可升级传统的Quil-A，并且这种基于复合佐剂的纳米疫苗作为商业疫苗的替代品在实际控制CE方面具有良好的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/31f581660ce6/IJN-20-309-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/697cbcef4983/IJN-20-309-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/4d9e5fac5a97/IJN-20-309-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/f0403c9018f4/IJN-20-309-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a7e8e83c43cf/IJN-20-309-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a6c726c07dd5/IJN-20-309-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a68ed7e0b905/IJN-20-309-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/b3b3e3b35a9d/IJN-20-309-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/31f581660ce6/IJN-20-309-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/697cbcef4983/IJN-20-309-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/4d9e5fac5a97/IJN-20-309-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/f0403c9018f4/IJN-20-309-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a7e8e83c43cf/IJN-20-309-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a6c726c07dd5/IJN-20-309-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/a68ed7e0b905/IJN-20-309-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/b3b3e3b35a9d/IJN-20-309-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/146e/11725278/31f581660ce6/IJN-20-309-g0008.jpg

相似文献

1
A Ferritin-Based Eg95 Nanoparticle Vaccine Adjuvanted with pCpG Eliciting Robust Immune Responses Against Cystic Echinococcosis in Mice Model.一种基于铁蛋白的Eg95纳米颗粒疫苗，佐以pCpG，在小鼠模型中引发针对囊型包虫病的强烈免疫反应。
Int J Nanomedicine. 2025 Jan 8;20:309-325. doi: 10.2147/IJN.S499938. eCollection 2025.
2
Synergistic effect of dual targeting vaccine adjuvant with aminated β-glucan and CpG-oligodeoxynucleotides for both humoral and cellular immune responses.氨基化β-葡聚糖与 CpG-寡脱氧核苷酸联合靶向疫苗佐剂对体液和细胞免疫应答的协同作用。
Acta Biomater. 2018 Sep 15;78:211-223. doi: 10.1016/j.actbio.2018.08.002. Epub 2018 Aug 9.
3
Functionalized aluminum hydroxide-based self-adjuvanted nanovaccine orchestrates antitumor immune responses via Dectin-1 signaling.功能化氢氧化铝基自佐剂纳米疫苗通过Dectin-1信号传导协调抗肿瘤免疫反应。
J Control Release. 2025 Feb 10;378:559-572. doi: 10.1016/j.jconrel.2024.12.035. Epub 2024 Dec 25.
4
Lipid nanoparticles as adjuvant of norovirus VLP vaccine augment cellular and humoral immune responses in a TLR9- and type I IFN-dependent pathway.脂质纳米颗粒作为诺如病毒VLP疫苗的佐剂，通过TLR9和I型干扰素依赖性途径增强细胞免疫和体液免疫反应。
J Virol. 2024 Dec 17;98(12):e0169924. doi: 10.1128/jvi.01699-24. Epub 2024 Nov 4.
5
Development of orf virus as a bifunctional recombinant vaccine: surface display of Echinococcus granulosus antigen EG95 by fusion to membrane structural proteins.ORF 病毒作为双功能重组疫苗的研制：通过融合到膜结构蛋白表面展示细粒棘球蚴抗原 EG95。
Vaccine. 2012 Jan 5;30(2):398-406. doi: 10.1016/j.vaccine.2011.10.079. Epub 2011 Nov 12.
6
Induction of Immunogenic Response in BALB/c Mice by Live and Killed Form of Recombinant Lactococcus lactis Displaying EG95 of Echinococcus granulosus.经重组乳球菌活疫苗和死疫苗诱导 BALB/c 小鼠产生免疫应答，展示细粒棘球绦虫 EG95 抗原。
Iran Biomed J. 2021 Jul 1;25(4):284-96. doi: 10.52547/ibj.25.4.284.
7
Adjuvant-free, self-assembling ferritin nanoparticle vaccine coupled with influenza virus hemagglutinin protein carrying M1 and PADRE epitopes elicits cross-protective immune responses.不含佐剂、自组装的铁蛋白纳米颗粒疫苗与携带M1和PADRE表位的流感病毒血凝素蛋白偶联，可引发交叉保护性免疫反应。
Front Immunol. 2025 Jan 31;16:1519866. doi: 10.3389/fimmu.2025.1519866. eCollection 2025.
8
PfCSP-ferritin nanoparticle malaria vaccine antigen formulated with aluminum-salt and CpG 1018® adjuvants: Preformulation characterization, antigen-adjuvant interactions, and mouse immunogenicity studies.用铝盐和CpG 1018®佐剂配制的PfCSP-铁蛋白纳米颗粒疟疾疫苗抗原：制剂前表征、抗原-佐剂相互作用及小鼠免疫原性研究
Hum Vaccin Immunother. 2025 Dec;21(1):2460749. doi: 10.1080/21645515.2025.2460749. Epub 2025 Feb 4.
9
A DNA vaccine (EG95-PT1/2/3-IL2) encoding multi-epitope antigen and IL-2 provokes efficient and long-term immunity to echinococcosis.一种 DNA 疫苗（EG95-PT1/2/3-IL2），可编码多表位抗原和 IL-2，可引发针对包虫病的有效和长期免疫。
J Control Release. 2023 Sep;361:402-416. doi: 10.1016/j.jconrel.2023.07.047. Epub 2023 Aug 12.
10
Pilot Field Trial of the EG95 Vaccine Against Ovine Cystic Echinococcosis in Rio Negro, Argentina: Second Study of Impact.阿根廷内乌肯针对绵羊囊型包虫病的EG95疫苗现场试验：影响的第二项研究
PLoS Negl Trop Dis. 2015 Oct 30;9(10):e0004134. doi: 10.1371/journal.pntd.0004134. eCollection 2015.

引用本文的文献

1
A tailored nanocarrier DMON and CpGs synergistically drive the formulation of a highly immunogenic and long-acting vaccine against echinococcosis.一种定制的纳米载体DMON和CpG协同推动针对棘球蚴病的高免疫原性长效疫苗的研制。
Mater Today Bio. 2025 May 12;32:101868. doi: 10.1016/j.mtbio.2025.101868. eCollection 2025 Jun.

本文引用的文献

1
CpG adjuvant enhances humoral and cellular immunity against OVA in different degrees in BALB/c, C57BL/6J, and C57BL/6N mice.CpG 佐剂在不同程度上增强了 BALB/c、C57BL/6J 和 C57BL/6N 小鼠对 OVA 的体液和细胞免疫反应。
Int Immunopharmacol. 2024 Sep 10;138:112593. doi: 10.1016/j.intimp.2024.112593. Epub 2024 Jul 6.
2
Engineered dendritic cells-derived exosomes harboring HIV-1 Nef-Tat fusion protein and heat shock protein 70: A promising HIV-1 safe vaccine candidate.工程化树突状细胞衍生的外泌体携带 HIV-1 Nef-Tat 融合蛋白和热休克蛋白 70：一种有前途的 HIV-1 安全疫苗候选物。
Int J Biol Macromol. 2024 Jun;270(Pt 2):132236. doi: 10.1016/j.ijbiomac.2024.132236. Epub 2024 May 18.
3
A DNA vaccine (EG95-PT1/2/3-IL2) encoding multi-epitope antigen and IL-2 provokes efficient and long-term immunity to echinococcosis.
一种 DNA 疫苗（EG95-PT1/2/3-IL2），可编码多表位抗原和 IL-2，可引发针对包虫病的有效和长期免疫。
J Control Release. 2023 Sep;361:402-416. doi: 10.1016/j.jconrel.2023.07.047. Epub 2023 Aug 12.
4
EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep.EgSeverin 和 Eg14-3-3zeta 来自细粒棘球绦虫，是用于犬和羊包虫病血清学诊断的潜在抗原。
Microb Pathog. 2023 Jun;179:106110. doi: 10.1016/j.micpath.2023.106110. Epub 2023 Apr 13.
5
Baculovirus-expressed self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induce protective immunity in mice.杆状病毒表达的靶向S蛋白的自组装新型冠状病毒纳米颗粒疫苗可诱导小鼠产生保护性免疫。
Process Biochem. 2023 Jun;129:200-208. doi: 10.1016/j.procbio.2023.03.026. Epub 2023 Mar 24.
6
A modular and self-adjuvanted multivalent vaccine platform based on porcine circovirus virus-like nanoparticles.基于猪圆环病毒样纳米颗粒的模块化自佐剂多价疫苗平台。
J Nanobiotechnology. 2022 Nov 24;20(1):493. doi: 10.1186/s12951-022-01710-4.
7
mRNA-encoded HIV-1 Env trimer ferritin nanoparticles induce monoclonal antibodies that neutralize heterologous HIV-1 isolates in mice.mRNA 编码的 HIV-1 Env 三聚体铁蛋白纳米颗粒诱导小鼠产生中和异种 HIV-1 分离株的单克隆抗体。
Cell Rep. 2022 Mar 15;38(11):110514. doi: 10.1016/j.celrep.2022.110514.
8
SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity.严重急性呼吸综合征冠状病毒2型铁蛋白纳米颗粒疫苗引发广泛的严重急性呼吸综合征冠状病毒免疫原性。
Cell Rep. 2021 Dec 21;37(12):110143. doi: 10.1016/j.celrep.2021.110143. Epub 2021 Dec 8.
9
Functionalizing Ferritin Nanoparticles for Vaccine Development.功能化铁蛋白纳米颗粒用于疫苗开发。
Pharmaceutics. 2021 Oct 5;13(10):1621. doi: 10.3390/pharmaceutics13101621.
10
Ferritin: A Multifunctional Nanoplatform for Biological Detection, Imaging Diagnosis, and Drug Delivery.铁蛋白：用于生物检测、成像诊断和药物输送的多功能纳米平台。
Acc Chem Res. 2021 Sep 7;54(17):3313-3325. doi: 10.1021/acs.accounts.1c00267. Epub 2021 Aug 20.