Xin Ting, Gao Xintao, Tao Siyi, Zhou Chenghao, Zhang Zhifang, Ding Jiabo, Ru Jiaxi, Li Yinü
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Mater Today Bio. 2025 May 12;32:101868. doi: 10.1016/j.mtbio.2025.101868. eCollection 2025 Jun.
Hydatid disease (echinococcosis) is a zoonotic parasitic disease that seriously endangers human health and livestock production. To develop a safer, more effective vaccine with an exceptionally long-lasting immune response, we employed an 'all-in-one' strategy to construct a nanovaccine against echinococcosis. In this system, a dendritic mesoporous organosilica nanoparticle (DMON), Eg95 antigen, and two types of CpG potentiators (CpG ODN and pCpG) were integrated into a single nanoplatform. Compared to the commercial Quil-A-formulated vaccine, these two nanovaccines exhibited significant advantages in inducing early, robust, and long-lasting protective immune responses, especially in terms of IgG1 antibody responses and Th1 cytokine TNF-α secretion. Notably, the certain adjuvant combination (DMON + pCpG) formulated-vaccine Eg95N + pCpG@DMON conferred stronger adjuvanticity to the antigen than Quil-A during the late stage (42-84 days). Systematic evaluation demonstrated excellent biodegradability and biosafety of DMON and its-based vaccine. This research provides a strong foundation for upgrading vaccines against echinococcosis.
包虫病（棘球蚴病）是一种严重危害人类健康和畜牧业生产的人畜共患寄生虫病。为了开发一种具有超长免疫应答的更安全、更有效的疫苗，我们采用了一种“一体化”策略来构建一种抗包虫病的纳米疫苗。在该系统中，树枝状介孔有机硅纳米颗粒（DMON）、Eg95抗原和两种CpG增强剂（CpG ODN和pCpG）被整合到一个单一的纳米平台中。与商业化的Quil-A配方疫苗相比，这两种纳米疫苗在诱导早期、强烈且持久的保护性免疫应答方面表现出显著优势，尤其是在IgG1抗体应答和Th1细胞因子TNF-α分泌方面。值得注意的是，在后期（42 - 84天），特定佐剂组合（DMON + pCpG）配方的疫苗Eg95N + pCpG@DMON赋予抗原的佐剂效应比Quil-A更强。系统评估表明DMON及其基疫苗具有出色的生物降解性和生物安全性。本研究为升级抗包虫病疫苗奠定了坚实基础。
