Wang Yiran, Wang Kaili, Yang Qiya, Wang Zhaoting, Su Yingying, Chen Xifei, Zhang Hongyin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Hortic Res. 2024 Sep 20;12(1):uhae264. doi: 10.1093/hr/uhae264. eCollection 2025 Jan.
Gene transcription is governed by a complex regulatory system involving changes in chromatin structure, the action of transcription factors, and the activation of -regulatory elements. Postharvest fruits are threatened by , a leading causal agent of blue mold disease and one of the most economically significant postharvest pathogens worldwide. However, information on its transcription regulatory mechanism is lagging. Here, we conducted an assay for transposase accessible chromatin sequencing (ATAC-seq) for during vegetative growth and infection phase and then studied the function of a basic leucine zipper (bZIP) transcription factor PeAtf1. Results highlighted the role of promoter regions in gene transcription and the significant difference in between these two phases. Six footprint-supported -regulatory elements of active transcription factors were obtained and analyzed. We then identified a homolog of the bZIP regulator Atf1, PeAtf1, and found it positively regulated vegetative growth, reproduction, and osmotic stress response in . Furthermore, deletion enhanced the fungus's tolerance to oxidative, cell wall, and membrane stresses, which might contribute to the virulence of deletion mutants in apple fruits, leading to similar pathogenicity between mutants and the wild type. Overall, this study provides new insights into the transcription regulatory profile of , aiding in the future development of strategies to control .
基因转录受一个复杂的调控系统支配，该系统涉及染色质结构的变化、转录因子的作用以及调控元件的激活。采后果实受到青霉的威胁，青霉是蓝霉病的主要致病因子，也是全球最具经济重要性的采后病原菌之一。然而，关于其转录调控机制的信息却很滞后。在此，我们对其在营养生长和感染阶段进行了转座酶可及染色质测序（ATAC-seq）分析，然后研究了一个碱性亮氨酸拉链（bZIP）转录因子PeAtf1的功能。结果突出了启动子区域在基因转录中的作用以及这两个阶段之间的显著差异。获得并分析了六个由足迹支持的活性转录因子的调控元件。然后我们鉴定出bZIP调节因子Atf1的一个同源物PeAtf1，并发现它正向调控其营养生长、繁殖和渗透胁迫响应。此外，缺失增强了该真菌对氧化、细胞壁和膜胁迫的耐受性，这可能导致缺失突变体在苹果果实中的毒力，使突变体和野生型之间具有相似的致病性。总体而言，本研究为其转录调控概况提供了新的见解，有助于未来防控策略的制定。
