Fatima Kiran, Naqvi Syed Zeeshan Haider, Ali Hazrat, Hassan Noor, Saqib Anam, Ansari Farheen, Saleem Sidrah, Jahan Shah, Ahmad Mushtaq
Department of Microbiology, Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore 54000, Pakistan.
Departemnt of Pathology, Rawalpindi Medical University, Rawalpindi 46000, Pakistan.
Data Brief. 2024 Dec 3;58:111188. doi: 10.1016/j.dib.2024.111188. eCollection 2025 Feb.
is a well-known opportunistic pathogen, responsible for various nosocomial infections. UOL-KIMZ-24 was previously isolated from a clinical specimen, collected from Lahore General Hospital, Lahore (LGH), Pakistan, dated 3rd March, 2022. During the initial screening for antimicrobial susceptibility, the UOL-KIMZ-24 was found a multiple drug resistant (MDR) strain. However, the detailed genomic insights for genes e.g. responsible for exhibiting antibiotic resistance via efflux pumps, have not yet been reported from strains, recovered from LGH. The current research to fills this gap by isolating, whole genome sequencing and subsequent post-sequencing analysis for addressing and identifying the efflux pumps associated genes, responsible for multiple drug resistant in . In a hybrid approach, short reads were processed through Illumina platform, while long reads were sequenced by MinION MK1B sequencing technique. The assembled and annotated genome of the UOL-KIMZ-24 revealed that it has 4048631 bp genome size with 179 contigs, 38.9 % GC content, 3628 protein coding sequences, 80 tRNA and 7 rRNA. The analysis of antibiotic-resistance genes (AMR) depicted 27 genes. where the genes encoding efflux pumps such as , and were the more prominent. In addition, sequence typing (ST) study showed that UOL-KIMZ-24 strain lies in ST2, six prophage sequences and 73 virulence factors were also identified in the studied UOL-KIMZ-24. Such an all-inclusive study uncovered the genetic flexibility of UOL-KIMZ-24 genome for acquiring MDR against in-practice antibiotics.
是一种著名的机会致病菌，可导致各种医院感染。UOL-KIMZ-24先前从一份临床标本中分离出来，该标本于2022年3月3日从巴基斯坦拉合尔的拉合尔综合医院（LGH）采集。在最初的抗菌药敏筛选中，发现UOL-KIMZ-24是一种多重耐药（MDR）菌株。然而，从LGH分离出的菌株中，尚未报道过例如通过外排泵表现出抗生素耐药性的基因的详细基因组见解。当前的研究通过分离、全基因组测序以及随后的测序后分析来填补这一空白，以解决和识别与外排泵相关的基因，这些基因导致了对……的多重耐药性。采用混合方法，短读长通过Illumina平台处理，而长读长则通过MinION MK1B测序技术进行测序。UOL-KIMZ-24的组装和注释基因组显示，其基因组大小为4048631 bp，有179个重叠群，GC含量为38.9%，有3628个蛋白质编码序列、80个tRNA和7个rRNA。抗生素耐药基因（AMR）分析显示有27个基因。其中编码外排泵的基因如……更为突出。此外，序列分型（ST）研究表明，UOL-KIMZ-24菌株属于ST2型，在所研究的UOL-KIMZ-24中还鉴定出6个前噬菌体序列和73个毒力因子。这样一项全面的研究揭示了UOL-KIMZ-24基因组在获得对实际应用抗生素的多重耐药性方面的遗传灵活性。
