Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University) (SIU), Lavale, Pune, Maharashtra, 412115, India.
Department of Food Science and Technology, University of California, Davis, USA.
Curr Microbiol. 2024 Nov 27;82(1):16. doi: 10.1007/s00284-024-04003-1.
Acinetobacter baumannii and Enterococcus faecalis are opportunistic bacteria frequently associated with hospital-acquired infections. A. baumannii nosocomial infections in intensive care units are a worldwide problem, with high mortality rates. It may also develop rapidly multidrug resistance (MDR), extensive drug resistance (XDR), and even pan-drug resistance (PDR). Colistin resistance which is an example of pan-drug resistance, is highly alarming as it's used as a last-line antibiotic. Microbes capable of crossing epithelial barriers such as E. faecalis have developed novel strategies to counter antimicrobial agents and cause bacteremia in immunocompromised patients. However, the coinfection of these bacteria in the same patient is unusual. Here, we report a genomic investigation of the extensively drug-resistant E. faecalis and A. baumannii isolated from the blood sample of a patient diagnosed with pauci-immune crescentic glomerulonephritis (PICGN). Identification of cultures isolated from blood sample was carried out using whole-genome sequencing and resistome profiles were mapped. Whole genome sequencing revealed that E. faecalis SVJ-EF01 had a genome size of 2,935,226 bp and GC content of 37.4%, whereas A. baumannii SVJ-AC01 had a genome size of 3,730,857 bp and GC content of 39%. Draft genomes were functionally annotated demonstrating that the organism harbors multiple virulence factors and antimicrobial-resistant mechanisms including MDR efflux pumps. A. baumannii genome possessed a CRISPR-Cas system which might contribute to antimicrobial resistance. This highlights the significance of polymicrobial nature in ESKAPE pathogenesis research. This genomic investigation helps to gain insights into the virulence, resistance profile, and functional potential of these pathogens.
鲍曼不动杆菌和粪肠球菌是经常与医院获得性感染相关的机会性细菌。重症监护病房的鲍曼不动杆菌医院感染是一个全球性问题，死亡率很高。它还可能迅速发展为多药耐药（MDR）、广泛耐药（XDR），甚至全耐药（PDR）。多粘菌素耐药是全耐药的一个例子，由于它被用作最后一线抗生素，因此非常令人警惕。能够穿过上皮屏障的微生物，如粪肠球菌，已经开发出了新的策略来对抗抗菌药物，并在免疫功能低下的患者中引起菌血症。然而，这些细菌在同一患者中的合并感染并不常见。在这里，我们报告了一例广泛耐药粪肠球菌和鲍曼不动杆菌的基因组研究，这些细菌是从一名诊断为少免疫性新月体性肾小球肾炎（PICGN）的患者的血液样本中分离出来的。使用全基因组测序和耐药组谱对从血液样本中分离的培养物进行了鉴定。全基因组测序显示，粪肠球菌 SVJ-EF01 的基因组大小为 2,935,226 bp，GC 含量为 37.4%，而鲍曼不动杆菌 SVJ-AC01 的基因组大小为 3,730,857 bp，GC 含量为 39%。草案基因组进行了功能注释，表明该生物具有多种毒力因子和抗菌药物耐药机制，包括 MDR 外排泵。鲍曼不动杆菌基因组具有 CRISPR-Cas 系统，这可能有助于抗菌药物耐药性。这突出了多微生物性质在 ESKAPE 发病机制研究中的重要性。这项基因组研究有助于深入了解这些病原体的毒力、耐药谱和功能潜力。
