He Cui, Ma Haidong, Zhang Tingting, Liu Yu, Zhang Cuiying, Deng Surong
Department of Blood Transfusion of Yong-chuan Hospital, Chongqing Medical University, Chongqing, 402160, People's Republic of China.
Department of Pharmacy of Yong-chuan Hospital, Chongqing Medical University, Chongqing, 402160, People's Republic of China.
J Blood Med. 2025 Jan 8;16:15-25. doi: 10.2147/JBM.S490649. eCollection 2025.
To study the platelet adhesion and aggregation behaviour of late pregnancy women under arterial shear rate using microfluidic chip technology and evaluate the risk of thrombosis in late pregnancy.
We included pregnant women who were registered in the obstetrics department of our hospital between January 2021 and October 2022 and underwent regular prenatal examinations. Blood samples were collected at 32-35 weeks of gestation for routine blood tests and progesterone, oestradiol, and platelet aggregation function. A microfluidic chip was used to construct an in vitro stenosis vascular model to explore the platelet reactivity at shear rates of 1000s-1, 1500s-1 and 4000s-1. Flow cytometry was used to analyse the effect of shear rate induction on the expression of platelet membrane surface fibrin receptor (PAC-1) and P-selectin (CD62P) in pregnant women.
Compared to the non-pregnant healthy control group, the white blood cell count increased and platelet count decreased significantly in late pregnant women (P < 0.05), and platelet reactivity to agonists increased under non-flow conditions (adhesion and aggregation rates, P < 0.05). Microfluidic chip technology showed that platelet aggregation in late pregnant women increased significantly (P < 0.05) in the shear-rate environment and was positively correlated with the shear rate. The degree of aggregation at 4000s was more evident, but the stability of platelet aggregates was low. Shear rate increased PAC-1 and CD62P expression.
Microfluidic chip technology was used to analyse the platelet aggregation function under arterial shear rate combined with flow cytometry to detect platelet activation, which was consistent with the traditional non-flow conditions used to evaluate platelet function. However, microfluidic technology can simulate a more realistic in vivo shear rate environment, providing more effective clinical application data and a theoretical basis for the diagnosis and prevention of platelet dysfunction and thrombotic diseases during pregnancy.
采用微流控芯片技术研究晚期妊娠妇女在动脉剪切率下的血小板黏附与聚集行为，并评估晚期妊娠血栓形成风险。
纳入2021年1月至2022年10月在我院产科登记并接受定期产前检查的孕妇。在妊娠32 - 35周采集血样进行血常规、孕酮、雌二醇及血小板聚集功能检测。使用微流控芯片构建体外狭窄血管模型，以探究在1000s⁻¹、1500s⁻¹和4000s⁻¹剪切率下的血小板反应性。采用流式细胞术分析剪切率诱导对孕妇血小板膜表面纤维蛋白受体（PAC - 1）和P - 选择素（CD62P）表达的影响。
与非妊娠健康对照组相比，晚期妊娠妇女白细胞计数升高，血小板计数显著降低（P < 0.05），在非流动条件下对激动剂的血小板反应性增加（黏附率和聚集率，P < 0.05）。微流控芯片技术显示，晚期妊娠妇女在剪切率环境下血小板聚集显著增加（P < 0.05），且与剪切率呈正相关。在4000s时聚集程度更明显，但血小板聚集体的稳定性较低。剪切率增加PAC - 1和CD62P表达。
利用微流控芯片技术分析动脉剪切率下的血小板聚集功能，并结合流式细胞术检测血小板活化，与传统用于评估血小板功能的非流动条件一致。然而，微流控技术可模拟更逼真的体内剪切率环境，为妊娠期血小板功能障碍和血栓性疾病的诊断与预防提供更有效的临床应用数据及理论依据。
