Rieger A W, Muir D C, Hendzel M R
J Assoc Off Anal Chem. 1985 Jan-Feb;68(1):59-61.
A simple method for determining picloram in fish is described. The sample is homogenized with ethyl acetate, acidified with 1N HCl, and extracted twice more with ethyl acetate. Ethyl acetate fractions are pooled, derivatized with diazomethane, cleaned up by column chromatography, and analyzed by electron capture gas chromatography. Rainbow trout exposed to 14C-picloram were used to evaluate the efficiency of 2 methods of extraction and to provide data on the rate of uptake and the bioconcentration factor. The detection limit for this method is 5 ng/g, using a 4 g sample.
本文描述了一种测定鱼类中毒莠定的简单方法。将样品用乙酸乙酯匀浆，用1N盐酸酸化，再用乙酸乙酯萃取两次。合并乙酸乙酯馏分，用重氮甲烷衍生化，通过柱色谱法净化，并用电子捕获气相色谱法分析。用暴露于14C-毒莠定的虹鳟鱼来评估两种萃取方法的效率，并提供摄取速率和生物浓缩因子的数据。该方法的检测限为5 ng/g，使用4 g样品。
