早产女性血浆中差异表达的非编码RNA的鉴定
Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth.
作者信息
Khan Waqasuddin, Kanwar Samiah, Mannan Mohammad Mohsin, Kabir Furqan, Iqbal Naveed, Nadeem Rajab Ali Mehdia, Zia Syeda Rehana, Mian Sharmeen, Aziz Fatima, Muneer Sahrish, Kalam Adil, Hussain Akram, Javed Iqra, Qazi Muhammad Farrukh, Khalid Javairia, Nisar Muhammad Imran, Jehan Fyezah
机构信息
Biorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, Pakistan.
Infectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, Pakistan.
出版信息
RNA Biol. 2025 Dec;22(1):1-8. doi: 10.1080/15476286.2024.2449278. Epub 2025 Jan 13.
This study aimed to identify differentially expressed non-coding RNAs (ncRNAs) associated with preterm birth (PTB) and determine biological pathways being influenced in the context of PTB. We processed cell-free RNA sequencing data and identified seventeen differentially expressed (DE) ncRNAs that could be involved in the onset of PTB. Per the validation via customized RT-qPCR, the recorded variations in expressions of eleven ncRNAs were concordant with the analyses. The results of this study provide insights into the role of DE ncRNAs and their impact on pregnancy-related biological pathways that could lead to PTB. Further studies are required to elucidate the precise mechanisms by which these DE ncRNAs contribute to adverse pregnancy outcomes (APOs) and their potential as diagnostic biomarkers.
本研究旨在鉴定与早产（PTB）相关的差异表达非编码RNA（ncRNA），并确定在PTB背景下受影响的生物学途径。我们处理了游离RNA测序数据，并鉴定出17种可能参与PTB发病的差异表达（DE）ncRNA。通过定制的RT-qPCR进行验证，11种ncRNA表达的记录变化与分析结果一致。本研究结果为DE ncRNA的作用及其对可能导致PTB的妊娠相关生物学途径的影响提供了见解。需要进一步研究以阐明这些DE ncRNA导致不良妊娠结局（APO）的精确机制及其作为诊断生物标志物的潜力。
Zotero 插件