Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, US Department of Health and Human Services, Detroit, MI 48201, USA.
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201 USA.
Cell Rep Med. 2021 Jun 15;2(6):100323. doi: 10.1016/j.xcrm.2021.100323.
Identification of pregnancies at risk of preterm birth (PTB), the leading cause of newborn deaths, remains challenging given the syndromic nature of the disease. We report a longitudinal multi-omics study coupled with a DREAM challenge to develop predictive models of PTB. The findings indicate that whole-blood gene expression predicts ultrasound-based gestational ages in normal and complicated pregnancies (r = 0.83) and, using data collected before 37 weeks of gestation, also predicts the delivery date in both normal pregnancies (r = 0.86) and those with spontaneous preterm birth (r = 0.75). Based on samples collected before 33 weeks in asymptomatic women, our analysis suggests that expression changes preceding preterm prelabor rupture of the membranes are consistent across time points and cohorts and involve leukocyte-mediated immunity. Models built from plasma proteomic data predict spontaneous preterm delivery with intact membranes with higher accuracy and earlier in pregnancy than transcriptomic models (AUROC = 0.76 versus AUROC = 0.6 at 27-33 weeks of gestation).
由于早产 (PTB) 的疾病具有综合征特征，因此识别有早产风险的妊娠（PTB 是新生儿死亡的主要原因）仍然具有挑战性。我们报告了一项纵向多组学研究，并结合 DREAM 挑战赛来开发 PTB 的预测模型。研究结果表明，全血基因表达可预测正常和复杂妊娠的基于超声的妊娠年龄（r=0.83），并且使用在 37 周妊娠之前收集的数据，还可预测正常妊娠（r=0.86）和自发性早产（r=0.75）的分娩日期。基于无症状妇女在 33 周前采集的样本，我们的分析表明，在早产前胎膜早破之前发生的表达变化在时间点和队列之间是一致的，并且涉及白细胞介导的免疫。基于血浆蛋白质组学数据构建的模型比转录组学模型更早、更准确地预测无膜自发性早产（AUROC=0.76 与 27-33 周时的 AUROC=0.6）。
