Mavroudakis Leonidas, Golubova Anastasia, Lanekoff Ingela
Department of Chemistry-BMC, Uppsala University, 75123, Uppsala, Sweden.
Department of Chemistry-BMC, Uppsala University, 75123, Uppsala, Sweden; Center of Excellence for the Chemical Mechanisms of Life, Uppsala University, Sweden.
Talanta. 2025 May 1;286:127460. doi: 10.1016/j.talanta.2024.127460. Epub 2025 Jan 10.
Spatial metabolomics offers the combination of molecular identification and localization. As a tool for spatial metabolomics, mass spectrometry imaging (MSI) can provide detailed information on localization. However, molecular annotation with MSI is challenging due to the lack of separation prior to mass spectrometric analysis. Contrarily, surface sampling capillary electrophoresis mass spectrometry (SS-CE-MS) provides detailed molecular information, although the size of the sampling sites is modest. Here, we describe a platform for spatial metabolomics where MSI using pneumatically assisted nanospray desorption electrospray ionization (PA-nano-DESI) is combined with SS-CE-MS to gain both in-depth chemical information and spatial localization from thin tissue sections. We present the workflow, including the user-friendly setup and switching between the techniques, compare the obtained data, and demonstrate a quantitative approach when using the platform for spatial metabolomics of ischemic stroke.
空间代谢组学提供了分子鉴定和定位的结合。作为空间代谢组学的一种工具，质谱成像（MSI）可以提供关于定位的详细信息。然而，由于在质谱分析之前缺乏分离步骤，利用MSI进行分子注释具有挑战性。相反，表面采样毛细管电泳质谱（SS-CE-MS）提供了详细的分子信息，尽管采样位点的尺寸适中。在此，我们描述了一种空间代谢组学平台，其中使用气动辅助纳米喷雾解吸电喷雾电离（PA-nano-DESI）的MSI与SS-CE-MS相结合，以从薄组织切片中获得深入的化学信息和空间定位。我们展示了工作流程，包括用户友好的设置和技术之间的切换，比较了获得的数据，并展示了在将该平台用于缺血性中风的空间代谢组学时的定量方法。
