Zhao Lulu, Peng Yongbo, Huang Jing, Liu Nishang, Zou Xinrong, Li Junnan, Fan Yunpeng, Li Ping, Tang Liling, Wang Jisheng, Zeng Yajun, Wu Yi, Zhu Gaohui
The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Key Laboratory for Pharmaceutical Metabolism Research, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
Department of Endocrinology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.
Int J Biol Macromol. 2025 Mar;296:139808. doi: 10.1016/j.ijbiomac.2025.139808. Epub 2025 Jan 11.
Polysaccharides are the major bioactive composition of Polygonatum sibiricum (P. sibiricum). However, the structural and functional identifications of these polysaccharides were still limited. Herein, we isolated a novel P. sibiricum polysaccharides (PSPF) and explored its potential function and mechanism in alleviating hyperlipidemia. PSPF were purified by diethylaminoethyl-sepharose fast flow (DEAE-Sepharose FF) and cross-linked dextran gel LH-20 (Sephadex LH-20) column chromatography, and identified by gel-permeation chromatography, methylation analysis, fourier transform infrared spectrometer (FT-IR), nuclear magnetic resonance (NMR), and gas chromatography-mass spectrometry (GC-MS). Their molecular weight (Mw), polysaccharide composition, and chemical structure were characterized. Furthermore, egg yolk emulsion-induced acute hyperlipidemia mouse model was constructed to evaluate the lipid-lowering efficacy and the underlying mechanism of PSPF. It was found that PSPF, with the Mw of 3592 Da, were prepared and mainly consisted of fructan with →1)-β-D-Fruf-(2 → main chain and →6)-β-D-Fruf-(2 → side chains. In addition, PSPF supplements efficiently reduced liver lipid accumulation, alleviated hepatocyte steatosis, and upregulated the AMP-activated protein kinase (AMPK) pathway, thereby enhancing fatty acid oxidation and decomposition. These results indicate that PSPF may serve as the potential dietary supplements for lipid reduction.
多糖是黄精的主要生物活性成分。然而，这些多糖的结构和功能鉴定仍然有限。在此，我们分离出一种新型黄精多糖（PSPF），并探讨其在缓解高脂血症方面的潜在功能和机制。PSPF通过二乙氨基乙基-琼脂糖快速流动柱（DEAE-琼脂糖FF）和交联葡聚糖凝胶LH-20柱色谱法纯化，并通过凝胶渗透色谱法、甲基化分析、傅里叶变换红外光谱仪（FT-IR）、核磁共振（NMR）和气相色谱-质谱联用仪（GC-MS）进行鉴定。对其分子量（Mw）、多糖组成和化学结构进行了表征。此外，构建了蛋黄乳液诱导的急性高脂血症小鼠模型，以评估PSPF的降脂效果和潜在机制。结果发现，制备的PSPF分子量为3592 Da，主要由具有→1)-β-D-呋喃果糖-(2→主链和→6)-β-D-呋喃果糖-(2→侧链的果聚糖组成。此外，PSPF补充剂有效地减少了肝脏脂质积累，减轻了肝细胞脂肪变性，并上调了AMP激活的蛋白激酶（AMPK）途径，从而增强了脂肪酸的氧化和分解。这些结果表明，PSPF可能作为潜在的降脂膳食补充剂。
