Tong Linjing, Huang Siming, Chen Guosheng, Ouyang Gangfeng
Sun Yat-sen University MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Guangzhou 510275, China.
Guangzhou Medical University Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou 511436, China.
Angew Chem Int Ed Engl. 2025 Feb 17;64(8):e202421192. doi: 10.1002/anie.202421192. Epub 2025 Jan 23.
Integrating enzymes with reticular frameworks offers promising avenues for access to functionally tailorable biocatalysis. This Minireview explores recent advances in enzyme-reticular framework hybrid biocomposites, focusing on the utilization of porous reticular frameworks, including metal-organic frameworks, covalent-organic frameworks, and hydrogen-bonded organic frameworks, to regulate the reactivity of an enzyme encapsulated inside mainly by pore infiltration and in situ encapsulation strategies. We highlight how pore engineering and host-guest interfacial interactions within reticular frameworks create tailored microenvironments that substantially impact the mass transfer and enzyme conformation, leading to biocatalytic rate enhancement, or imparting enzymes with non-native biocatalytic functions, including substrate selectivity and new activity. Additionally, the feasibility of leveraging the photothermal effect of a framework to optimize the local reaction temperature and photoelectric effect to elicit diverse photoenzyme-coupled reactions is also summarized in detail, which can expand the functional repertoire of biocatalytic transformations under light irradiation. This Minireview underscores the potential of reticular frameworks as tunable and reliable platforms to govern biocatalysis, offering pathways for engineering sustainable, efficient, and selective biocatalytic reactors in pharmaceutical, environmental, and energy-related applications.
将酶与网状框架相结合为实现功能可定制的生物催化提供了有前景的途径。本综述探讨了酶-网状框架杂化生物复合材料的最新进展，重点关注多孔网状框架的应用，包括金属有机框架、共价有机框架和氢键有机框架，主要通过孔渗透和原位封装策略来调节包裹在其中的酶的反应活性。我们强调了网状框架内的孔工程和主客体界面相互作用如何创造定制的微环境，这对传质和酶构象产生重大影响，从而提高生物催化速率，或赋予酶非天然的生物催化功能，包括底物选择性和新活性。此外，还详细总结了利用框架的光热效应优化局部反应温度以及利用光电效应引发多种光酶偶联反应的可行性，这可以扩展光照射下生物催化转化的功能范围。本综述强调了网状框架作为调控生物催化的可调谐且可靠平台的潜力，为在制药、环境和能源相关应用中设计可持续、高效且选择性的生物催化反应器提供了途径。
