Sharew Sisay Gebrish, Weldehanna Daniel Gebretsadik, Gebreyes Demissew Shenkute, Abebe Tsegahun Asfaw, Shibabaw Agumas
Department of Medical Laboratory Science, Debere Birhan Health Science College. Debre, Berhan, Ethiopia.
Department of Medical Laboratory Science, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Sci Rep. 2025 Jan 13;15(1):1784. doi: 10.1038/s41598-024-84563-1.
Extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing (CP) gram-negative bacteria are the major public health concerns. Gowns used by healthcare workers (HCWs) in daily practice are a source of hospital-acquired infections in hospital settings. The study aimed to determine the prevalence of extended-spectrum beta-lactamase and carbapenemase-producing gram-negative bacteria from gowns of healthcare workers at Debre Berhan Comprehensive Specialized Hospital, Amhara Regional State, Ethiopia. A hospital-based cross-sectional study was conducted from August to October 2022. A total of 226 swab samples were collected from gowns of HCWs and cultured on MacConkey agar. Bacterial identifications were done by standard biochemical tests. Screening for ESBL and carbapenemase production was done using CHROME agar. Confirmation of ESBL and carbapenemase production was done by the combination disk method and modified carbapenem inactivation method, respectively. Data were analyzed by using SPSS version 25. The overall contamination rate of gowns was 46.9% (106/226). Among 226 swab samples, a total of 117 (51.7%) gram-negative bacteria were isolated. Among these, the most frequent isolates were E. coli, accounted 36 (30.8%) followed by K. pneumoniae, 26(22.2%). The overall multi-drug resistance (MDR) rate was 65 (55.6%). Of the 117 isolates, 17(14.5%) and 12(10.3%) were ESBL and carbapenemase producers respectively. Gown type (p = 0.041), laundering practice (p = 0.045), number of gowns (p = 0.002), and gown washing frequency per week (p = 0.017).were significantly associated with bacterial contamination of gowns. In this study, the prevalence of ESBL and CP Gram-negative bacteria from gowns of healthcare workers was found to be alarming. Therefore, strict infection prevention and control practices, as well as good hygienic practices, should be implemented to reduce and prevent cross-contamination and the spread of antimicrobial-resistant strains in hospital settings.
产超广谱β-内酰胺酶（ESBL）和碳青霉烯酶（CP）的革兰氏阴性菌是主要的公共卫生问题。医护人员在日常工作中使用的工作服是医院环境中医院获得性感染的一个来源。本研究旨在确定埃塞俄比亚阿姆哈拉州德布雷伯汉综合专科医院医护人员工作服中产超广谱β-内酰胺酶和碳青霉烯酶的革兰氏阴性菌的流行情况。2022年8月至10月进行了一项基于医院的横断面研究。共从医护人员的工作服上采集了226份拭子样本，并在麦康凯琼脂上进行培养。通过标准生化试验进行细菌鉴定。使用CHROME琼脂对ESBL和碳青霉烯酶的产生进行筛查。分别通过复合纸片法和改良碳青霉烯灭活法对ESBL和碳青霉烯酶的产生进行确认。使用SPSS 25版对数据进行分析。工作服的总体污染率为46.9%（106/226）。在226份拭子样本中，共分离出117株（51.7%）革兰氏阴性菌。其中，最常见的分离株是大肠杆菌，占36株（30.8%），其次是肺炎克雷伯菌，26株（22.2%）。总体多重耐药（MDR）率为65株（55.6%）。在117株分离株中，分别有17株（14.5%）和12株（10.3%）产ESBL和碳青霉烯酶。工作服类型（p = 0.041）、洗涤方式（p = 0.045）、工作服数量（p = 0.002）和每周工作服洗涤频率（p = 0.017）与工作服的细菌污染显著相关。在本研究中，发现医护人员工作服中产ESBL和CP的革兰氏阴性菌的流行情况令人担忧。因此，应实施严格的感染预防和控制措施以及良好的卫生习惯，以减少和防止医院环境中的交叉污染和抗菌药物耐药菌株的传播。
