用于临床研究的高效液相色谱柱性能评估。

Evaluation of HPLC column performance for clinical studies.

作者信息

Simpson R C, Brown P R, Schwartz M K

出版信息

J Chromatogr Sci. 1985 Mar;23(3):89-94. doi: 10.1093/chromsci/23.3.89.

DOI:10.1093/chromsci/23.3.89

Abstract

Several C18 HPLC columns, varying in column length as well as packing material diameter, were used to separate mixtures of biochemical compounds in standards and in various physiological fluids. The column performances were compared both qualitatively and quantitatively. Factors investigated included efficiency, resolution, selectivity, column effect on peak height, temperature effects, cost per analysis, and analysis times. Column lifetime was also examined. The results were used to evaluate the suitability of the various columns for use in the chromatographic analysis of physiological samples.

摘要

使用了几根C18高效液相色谱柱，这些柱子在柱长以及填料直径方面各不相同，用于分离标准品和各种生理流体中的生化化合物混合物。对柱性能进行了定性和定量比较。研究的因素包括柱效、分离度、选择性、柱对峰高的影响、温度影响、每次分析的成本以及分析时间。还考察了柱寿命。结果用于评估各种柱子在生理样品色谱分析中的适用性。