Simpson R C, Brown P R
Department of Chemistry, University of Rhode Island, Kingston 02881.
J Chromatogr. 1987 Jul 29;400:297-305. doi: 10.1016/s0021-9673(01)81625-3.
Fast high-performance liquid chromatography (HPLC) (5-10 cm X 4.6 mm I.D. columns), microbore HPLC (25 cm X 2.1 mm and 1 mm I.D. columns), and fast microbore HPLC (5-10 cm X 2.1 mm and 1 mm I.D. columns) were successfully applied to the separation of nucleic acid constituents in standard mixtures and physiological fluids. Separations were obtained in isocratic and gradient elution modes. The separations obtained were compared with those achieved on a conventional 25 cm X 4.6 mm I.D. column. Factors evaluated included separation time, retention time reproducibility, peak height reproducibility, resolution, efficiency, sensitivity and linear response range. Practical factors, such as the amount of sample required and cost per analysis, were also examined.
快速高效液相色谱法（HPLC）（内径5 - 10厘米×4.6毫米的色谱柱）、微径HPLC（内径25厘米×2.1毫米和1毫米的色谱柱）以及快速微径HPLC（内径5 - 10厘米×2.1毫米和1毫米的色谱柱）已成功应用于标准混合物和生理体液中核酸成分的分离。分离在等度洗脱和梯度洗脱模式下进行。将所获得的分离结果与在传统的内径25厘米×4.6毫米色谱柱上获得的结果进行比较。评估的因素包括分离时间、保留时间重现性、峰高重现性、分离度、效率、灵敏度和线性响应范围。还考察了实际因素，如所需样品量和每次分析的成本。
