Simpson R C, Brown P R
J Chromatogr. 1987 Jan 9;385:41-54. doi: 10.1016/s0021-9673(01)94620-5.
Simple and relatively inexpensive modifications are described for conversion of an existing high-performance liquid chromatographic system to a microbore system capable of isocratic or gradient elution. Use of micro guard columns was shown to be a practical method of protecting the microbore analytical columns when chromatographing samples of physiological origin. The microbore chromatograph was successfully integrated with an autoinjector to permit automation of the system. The modified microbore system was used successfully for the isocratic and gradient separations of standard solutions as well as physiological samples. Evaluation of the system performance was based upon comparisons of column efficiency, selectivity, resolution, and sensitivity.
本文描述了一些简单且相对廉价的改进方法，可将现有的高效液相色谱系统转换为能够进行等度洗脱或梯度洗脱的微径系统。当对生理来源的样品进行色谱分析时，使用微保护柱被证明是保护微径分析柱的一种实用方法。该微径色谱仪成功地与自动进样器集成，实现了系统的自动化。改进后的微径系统成功地用于标准溶液以及生理样品的等度和梯度分离。系统性能的评估基于柱效、选择性、分离度和灵敏度的比较。
