Yuan Xing-Yue, Xiao Han, Wu Li, Zhao Jie, Wu Jia-Rui, Ni Yu, Xiao Xin, Yang Hai-Ping
Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang, 550025, China.
Department of Nephrology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatric Metabolism and Inflammatory Diseases, Chongqing, 400042, China.
Chemistry. 2025 Mar 12;31(15):e202404257. doi: 10.1002/chem.202404257. Epub 2025 Jan 13.
In this study, a supramolecular fluorescent material was constructed by using double-cavity twisted cucurbit[14]uril (tQ[14]) and positively charged Astrazon Pink FG (APFG) based on the non-covalent host-guest interaction for the first time. The thermodynamic parameters of the APFG@tQ[14] in aqueous solution were determined by isothermal titration calorimetry (ITC), the results indicated that the spontaneous assembly of APFG@tQ[14] is mainly driven by enthalpy. The intramolecular charge transfer (ICT) effect induced the APFG@tQ[14] probe to emit a strong orange-red fluorescence. Due to its good optical properties and low toxicity, APFG@tQ[14] was successfully applied to multi-channel imaging of HK2 cells. This demonstrates the great potential of APFG@tQ[14] in labeling and tracking living cells.
在本研究中，首次基于非共价主客体相互作用，利用双腔扭曲葫芦[14]脲（tQ[14]）和带正电荷的阿斯特拉宗粉红FG（APFG）构建了一种超分子荧光材料。通过等温滴定量热法（ITC）测定了APFG@tQ[14]在水溶液中的热力学参数，结果表明APFG@tQ[14]的自发组装主要由焓驱动。分子内电荷转移（ICT）效应促使APFG@tQ[14]探针发出强烈的橙红色荧光。由于其良好的光学性质和低毒性，APFG@tQ[14]成功应用于HK2细胞的多通道成像。这证明了APFG@tQ[14]在标记和追踪活细胞方面具有巨大潜力。
