Hussain Shazia Fathima Jaffer, Abullais Shahabe Saquib, Bottu Kavitha, Thirumani Logalakshmi, Misbah Iffath, Madar Inamul Hasan, Alghamdi Nuha S, Karobari Mohmed Isaqali
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu 600077, India.
Department of Oral Pathology and Microbiology, Meenakshi Ammal Dental College and Hospitals, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu 600095, India.
Oncol Lett. 2025 Jan 3;29(3):115. doi: 10.3892/ol.2025.14862. eCollection 2025 Mar.
The present study investigated the involvement of human papillomavirus (HPV)16 and HPV18 in oropharyngeal malignancies in order to understand the oncogenic mechanisms, and to identify biomarkers for early detection and treatment targets. Given the rising incidence of HPV-associated cancer, particularly in India, this holds significance in elucidating the molecular basis of these diseases. Structural validation of HPV16 and 18 oncoproteins E6 and E7 was conducted using computational tools, while gene expression profiles related to oral squamous cell carcinoma (OSCC) were analyzed to assess differential expression. The presence of HPV in patient tissue sections was examined using reverse transcription-PCR. The present study revealed the interactions of HPV16 and 18 E6/E7 oncoproteins, highlighting their role in cancer progression by targeting key tumor suppressors, such as p53 and retinoblastoma protein. Further analysis demonstrated the involvement of HPV16 and 18 E6/E7 oncoproteins in cancer pathways, signaling and telomere regulation, which supports the development of future targeted therapies. HPV16 and 18 E6/E7 represent promising therapeutic targets in OSCC, and provide further insights into potential diagnostic and treatment avenues. The present study contributes to the current understanding of HPV-associated cancer and innovative strategies in disease management.
本研究调查了人乳头瘤病毒（HPV）16型和18型与口咽恶性肿瘤的关系，以了解其致癌机制，并确定早期检测的生物标志物和治疗靶点。鉴于HPV相关癌症的发病率不断上升，尤其是在印度，这对于阐明这些疾病的分子基础具有重要意义。使用计算工具对HPV16和18型癌蛋白E6和E7进行了结构验证，同时分析了与口腔鳞状细胞癌（OSCC）相关的基因表达谱，以评估差异表达。使用逆转录PCR检测患者组织切片中HPV的存在情况。本研究揭示了HPV16和18型E6/E7癌蛋白的相互作用，突出了它们通过靶向关键肿瘤抑制因子（如p53和视网膜母细胞瘤蛋白）在癌症进展中的作用。进一步分析表明，HPV16和18型E6/E7癌蛋白参与了癌症通路、信号传导和端粒调节，这为未来靶向治疗的发展提供了支持。HPV16和18型E6/E7是OSCC中有前景的治疗靶点，并为潜在的诊断和治疗途径提供了进一步的见解。本研究有助于当前对HPV相关癌症的理解以及疾病管理中的创新策略。
