Saratale Ganesh Dattatraya, Saratale Rijuta Ganesh, Ghodake Gajanan, Shinde Surendra, Kim Dae-Young, Alyousef Abdullah A, Arshad Mohammed, Syed Asad, Pant Deepak, Shin Han-Seung
Department of Food Science and Biotechnology, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Korea.
Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Korea.
Nanomaterials (Basel). 2020 May 22;10(5):997. doi: 10.3390/nano10050997.
Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs-CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV-visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000-1,1000 and 120-480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.
氨基糖苷类药物（AMGs）已被广泛用于治疗家畜和人类由革兰氏阴性菌引起的传染病。基于金霉素包覆的银纳米颗粒（AgNPs-CTC）作为传感元件，提出了一种用于测定链霉素和卡那霉素的选择性和灵敏的比色探针。几乎所有测试的氨基糖苷类抗生素都能迅速诱导银纳米颗粒聚集，同时颜色从黄色变为橙色/红色。通过紫外（UV）光谱法可以实现对包括妥布霉素、链霉素、阿米卡星、庆大霉素、新霉素和卡那霉素在内的氨基糖苷类抗生素与其他类型抗生素的选择性检测。这种开发的比色测定法能够通过深入的表面等离子体共振（SPR）研究检测各种AMGs。通过使用紫外可见分光光度计，在皮摩尔水平（pM）实现了链霉素和卡那霉素的测定。在水相条件下，链霉素和卡那霉素比色传感器的线性范围分别为1000 - 11000和120 - 480 pM。相应的检测限分别为2000 pM和120 pM。因此，经过验证的双色比色和比率法可用于水样中AMG抗生素超灵敏和快速检测的各种分析应用。
