Caro René, Chen Andrew, Mudumbai Raghu, Duerr Eric, Chen Philip P, Bojikian Karine D
Department of Ophthalmology, University of Washington, Seattle, WA, United States of America.
PLoS One. 2025 Jan 14;20(1):e0317354. doi: 10.1371/journal.pone.0317354. eCollection 2025.
To investigate macula and optic nerve head (ONH) mitochondrial metabolic activity using flavoprotein fluorescence (FPF) in normal, glaucoma suspect (GS), and open-angle glaucoma (OAG) eyes we performed a cross-sectional, observational study of FPF in normal, GS, and OAG eyes. The macula and ONH of each eye was scanned and analyzed with a commercially available FPF measuring device (OcuMet Beacon, OcuSciences Inc., Ann Arbor, MI). One-way analysis of variance was used to compare macula and ONH FPF scores between groups. Linear regression models investigated the correlation between FPF scores and structural and functional parameters. We included 25 normal, 16 GS, and 54 OAG eyes. The average age in years ± SD for normal, GS, and OAG groups was 60.6 ±17.4, 67.8 ± 10.3, and 67.9 ± 11.6, respectively (P = 0.064). There was no significant difference in gender, race/ethnicity, visual acuity, and intraocular pressure between groups. OAG eyes had larger cup-to-disc ratio, thinner retinal nerve fiber and macula thicknesses, and worse visual field indices compared to normal and GS eyes (P ≤ 0.018). There was no significant difference in any FPF metric between the study groups in either the macula or the ONH, despite normalizing FPF data for structural differences between groups (e.g. retinal nerve fiber layer and ganglion cell-inner plexiform layer thickness). In conclusion, no significant differences in metabolic activity as measured by FPF were found in macula and ONH FPF scores using the integrated clinician report generator between normal, GS, and OAG eyes. Further research is needed to evaluate the role of mitochondrial metabolic activity measurements in glaucoma.
为了使用黄素蛋白荧光（FPF）研究正常眼、青光眼疑似患者（GS）眼和开角型青光眼（OAG）眼的黄斑和视神经乳头（ONH）线粒体代谢活性，我们对正常眼、GS眼和OAG眼的FPF进行了一项横断面观察性研究。使用市售的FPF测量设备（OcuMet Beacon，OcuSciences公司，密歇根州安阿伯）对每只眼睛的黄斑和ONH进行扫描和分析。采用单因素方差分析比较各组之间黄斑和ONH的FPF评分。线性回归模型研究了FPF评分与结构和功能参数之间的相关性。我们纳入了25只正常眼、16只GS眼和54只OAG眼。正常组、GS组和OAG组的平均年龄（岁±标准差）分别为60.6±17.4、67.8±10.3和67.9±11.6（P = 0.064）。各组之间在性别、种族/民族、视力和眼压方面无显著差异。与正常眼和GS眼相比，OAG眼的杯盘比更大、视网膜神经纤维和黄斑厚度更薄、视野指数更差（P≤0.018）。尽管对各组之间的结构差异（如视网膜神经纤维层和神经节细胞-内丛状层厚度）进行了FPF数据标准化，但研究组之间在黄斑或ONH的任何FPF指标上均无显著差异。总之，使用综合临床医生报告生成器测量的黄斑和ONH的FPF评分中，正常眼、GS眼和OAG眼之间在代谢活性方面未发现显著差异。需要进一步研究来评估线粒体代谢活性测量在青光眼中的作用。
