Polydopamine-Mediated, Centrifugal Force-Driven Gold Nanoparticle-Deposited Microneedle SERS Sensors for Food Safety Monitoring Theoretical Study of the SERS Substrate Fabrication.

Microneedle (MN) sensors have great promise for food safety detection, but the rapid preparation of MNs for surface-enhanced Raman scattering (SERS) sensors with tunable and homogeneous nanoparticles remains a great challenge. To address this, a SERS sensor of gold nanoparticles@polydopamine@poly(methyl methacrylate) MN (AuNPs@PDA@PMMA-MN) was developed. The extended-Derjaguin-Landau-Verwey-Overbeek theory was applied to calculate the interaction energy between AuNPs and PDA. It was confirmed that an appropriate centrifugal force could be utilized to overcome the electrostatic repulsion between AuNPs and PDA. Together with the adhesion force of PDA, AuNPs can therefore be uniformly and densely deposited on the MN substrate. The AuNPs@PDA@PMMA-MN had an enhancement factor of up to 1.74 × 10 for R6G. Furthermore, a MN sensor for the selective detection of putrescine and cadaverine was successfully constructed by modifying 4-mercaptobenzaldehyde (4-MBA) on AuNPs@PDA@PMMA-MN substrates. This sensor could quantitatively detect putrescine and cadaverine in meat. It has been successfully applied to the in situ detection of putrescine and cadaverine in real meat samples. The AuNPs@PDA@PMMA-MN SERS sensor has the advantages of facile fabrication, high sensitivity, high specificity, and online, in situ detection capability. It is expected to have applications in food quality testing, environmental monitoring, and disease diagnosis.