Yang Kejie, Zhong Feng, Peng Cheng, Wang Feng, Yang Haifeng
The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, P. R. China.
ACS Sens. 2025 Jan 24;10(1):339-349. doi: 10.1021/acssensors.4c02556. Epub 2025 Jan 14.
Microneedle (MN) sensors have great promise for food safety detection, but the rapid preparation of MNs for surface-enhanced Raman scattering (SERS) sensors with tunable and homogeneous nanoparticles remains a great challenge. To address this, a SERS sensor of gold nanoparticles@polydopamine@poly(methyl methacrylate) MN (AuNPs@PDA@PMMA-MN) was developed. The extended-Derjaguin-Landau-Verwey-Overbeek theory was applied to calculate the interaction energy between AuNPs and PDA. It was confirmed that an appropriate centrifugal force could be utilized to overcome the electrostatic repulsion between AuNPs and PDA. Together with the adhesion force of PDA, AuNPs can therefore be uniformly and densely deposited on the MN substrate. The AuNPs@PDA@PMMA-MN had an enhancement factor of up to 1.74 × 10 for R6G. Furthermore, a MN sensor for the selective detection of putrescine and cadaverine was successfully constructed by modifying 4-mercaptobenzaldehyde (4-MBA) on AuNPs@PDA@PMMA-MN substrates. This sensor could quantitatively detect putrescine and cadaverine in meat. It has been successfully applied to the in situ detection of putrescine and cadaverine in real meat samples. The AuNPs@PDA@PMMA-MN SERS sensor has the advantages of facile fabrication, high sensitivity, high specificity, and online, in situ detection capability. It is expected to have applications in food quality testing, environmental monitoring, and disease diagnosis.
微针（MN）传感器在食品安全检测方面具有巨大潜力，但快速制备具有可调谐且均匀纳米颗粒的用于表面增强拉曼散射（SERS）传感器的微针仍然是一个巨大挑战。为解决这一问题，开发了一种金纳米颗粒@聚多巴胺@聚（甲基丙烯酸甲酯）微针（AuNPs@PDA@PMMA-MN）的SERS传感器。应用扩展的Derjaguin-Landau-Verwey-Overbeek理论来计算金纳米颗粒与聚多巴胺之间的相互作用能。证实可以利用适当的离心力来克服金纳米颗粒与聚多巴胺之间的静电排斥。因此，连同聚多巴胺的粘附力，金纳米颗粒可以均匀且密集地沉积在微针基底上。AuNPs@PDA@PMMA-MN对罗丹明6G（R6G）的增强因子高达1.74×10⁶。此外，通过在AuNPs@PDA@PMMA-MN基底上修饰4-巯基苯甲醛（4-MBA），成功构建了一种用于选择性检测腐胺和尸胺的微针传感器。该传感器可以定量检测肉类中的腐胺和尸胺。它已成功应用于实际肉类样品中腐胺和尸胺的原位检测。AuNPs@PDA@PMMA-MN SERS传感器具有制备简便、灵敏度高、特异性高以及在线原位检测能力等优点。预计在食品质量检测、环境监测和疾病诊断等方面具有应用前景。
